Structural and functional damages of whole body ionizing radiation on rat brain homogenate membranes and protective effect of amifostine

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dc.contributor.authorArslan, Gülgün Çakmak
dc.contributor.authorSevercan, Mete
dc.contributor.authorZorlu, Faruk
dc.contributor.authorSevercan, Feride
dc.date.accessioned2020-04-30T23:32:15Z
dc.date.available2020-04-30T23:32:15Z
dc.date.issued2016
dc.departmentDÜ, Fen-Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionZorlu, Faruk/0000-0002-9835-4768; Cakmak-Arslan, Gulgun/0000-0003-4326-1780en_US
dc.descriptionWOS: 000388720200010en_US
dc.descriptionPubMed: 27585945en_US
dc.description.abstractPurpose: To investigate the effects of whole body ionizing radiation at a sublethal dose on rat brain homogenate membranes and the protective effects of amifostine on these systems at molecular level. Materials and methods: Sprague-Dawley rats, in the absence and presence of amifostine, were whole-body irradiated at a single dose of 8Gy and decapitated after 24 h. The brain homogenate membranes of these rats were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Results: Ionizing radiation caused a significant increase in the lipid to protein ratio and significant decreases in the ratios of olefinic = CH/lipid, CH2/lipid, carbonyl ester/lipid and CH3/lipid suggesting, respectively, a more excessive decrease in the protein content and the degradation of lipids as a result of lipid peroxidation. In addition, radiation changed the secondary structure of proteins and the status of packing of membrane lipid head groups. Furthermore, it caused a decrease in lipid order and an increase in membrane fluidity. The administration of amifostine before ionizing radiation inhibited all the radiation-induced alterations in brain homogenate membranes. Conclusions: The results revealed that whole body ionizing radiation at a sublethal dose causes significant alterations in the structure, composition and dynamics of brain homogenate membranes and amifostine has a protective effect on these membranes.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-2939]; Middle East Technical University (METU) [BAP-2006-07-02-0001]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technical Research Council of Turkey (TUBITAK), SBAG-2939, and by the Middle East Technical University (METU) Research Fund, BAP-2006-07-02-0001.en_US
dc.identifier.doi10.1080/09553002.2016.1230237en_US
dc.identifier.endpage848en_US
dc.identifier.issn0955-3002
dc.identifier.issn1362-3095
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage837en_US
dc.identifier.urihttps://doi.org/10.1080/09553002.2016.1230237
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4661
dc.identifier.volume92en_US
dc.identifier.wosWOS:000388720200010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofInternational Journal Of Radiation Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmifostineen_US
dc.subjectbrain homogenate membraneen_US
dc.subjectFTIR spectroscopyen_US
dc.subjectionizing radiationen_US
dc.subjectlipid peroxidationen_US
dc.subjectprotein secondary structureen_US
dc.titleStructural and functional damages of whole body ionizing radiation on rat brain homogenate membranes and protective effect of amifostineen_US
dc.typeArticleen_US

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