Structural and functional damages of whole body ionizing radiation on rat brain homogenate membranes and protective effect of amifostine
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis Ltd
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Purpose: To investigate the effects of whole body ionizing radiation at a sublethal dose on rat brain homogenate membranes and the protective effects of amifostine on these systems at molecular level. Materials and methods: Sprague-Dawley rats, in the absence and presence of amifostine, were whole-body irradiated at a single dose of 8Gy and decapitated after 24 h. The brain homogenate membranes of these rats were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Results: Ionizing radiation caused a significant increase in the lipid to protein ratio and significant decreases in the ratios of olefinic = CH/lipid, CH2/lipid, carbonyl ester/lipid and CH3/lipid suggesting, respectively, a more excessive decrease in the protein content and the degradation of lipids as a result of lipid peroxidation. In addition, radiation changed the secondary structure of proteins and the status of packing of membrane lipid head groups. Furthermore, it caused a decrease in lipid order and an increase in membrane fluidity. The administration of amifostine before ionizing radiation inhibited all the radiation-induced alterations in brain homogenate membranes. Conclusions: The results revealed that whole body ionizing radiation at a sublethal dose causes significant alterations in the structure, composition and dynamics of brain homogenate membranes and amifostine has a protective effect on these membranes.
Description
Zorlu, Faruk/0000-0002-9835-4768; Cakmak-Arslan, Gulgun/0000-0003-4326-1780
WOS: 000388720200010
PubMed: 27585945
WOS: 000388720200010
PubMed: 27585945
Keywords
Amifostine, brain homogenate membrane, FTIR spectroscopy, ionizing radiation, lipid peroxidation, protein secondary structure
Journal or Series
International Journal Of Radiation Biology
WoS Q Value
Q1
Scopus Q Value
Q2
Volume
92
Issue
12
