Structural and functional damages of whole body ionizing radiation on rat brain homogenate membranes and protective effect of amifostine
Yükleniyor...
Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: To investigate the effects of whole body ionizing radiation at a sublethal dose on rat brain homogenate membranes and the protective effects of amifostine on these systems at molecular level. Materials and methods: Sprague-Dawley rats, in the absence and presence of amifostine, were whole-body irradiated at a single dose of 8Gy and decapitated after 24 h. The brain homogenate membranes of these rats were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Results: Ionizing radiation caused a significant increase in the lipid to protein ratio and significant decreases in the ratios of olefinic = CH/lipid, CH2/lipid, carbonyl ester/lipid and CH3/lipid suggesting, respectively, a more excessive decrease in the protein content and the degradation of lipids as a result of lipid peroxidation. In addition, radiation changed the secondary structure of proteins and the status of packing of membrane lipid head groups. Furthermore, it caused a decrease in lipid order and an increase in membrane fluidity. The administration of amifostine before ionizing radiation inhibited all the radiation-induced alterations in brain homogenate membranes. Conclusions: The results revealed that whole body ionizing radiation at a sublethal dose causes significant alterations in the structure, composition and dynamics of brain homogenate membranes and amifostine has a protective effect on these membranes.
Açıklama
Zorlu, Faruk/0000-0002-9835-4768; Cakmak-Arslan, Gulgun/0000-0003-4326-1780
WOS: 000388720200010
PubMed: 27585945
WOS: 000388720200010
PubMed: 27585945
Anahtar Kelimeler
Amifostine, brain homogenate membrane, FTIR spectroscopy, ionizing radiation, lipid peroxidation, protein secondary structure
Kaynak
International Journal Of Radiation Biology
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
92
Sayı
12
