Assessment of markers expressed in human hair follicles according to different skin regions

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dc.contributor.authorYay, Arzu
dc.contributor.authorGöktepe, Özge
dc.contributor.authorBahadır, Anzel
dc.contributor.authorÖzdamar, Saim
dc.contributor.authorÖktem, İbrahim Suat
dc.contributor.authorÇoruh, Atilla
dc.contributor.authorBaran, Münevver
dc.date.accessioned2020-04-30T22:39:46Z
dc.date.available2020-04-30T22:39:46Z
dc.date.issued2018
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionCoruh, Atilla/0000-0002-3592-3433en_US
dc.descriptionWOS: 000440357400010en_US
dc.descriptionPubMed: 29962115en_US
dc.description.abstractBackground. Body region-dependent hair follicle (HF) characteristics are concerned with follicular size and distribution, and have been demonstrated to have characteristics for each region of the body. Objectives. The aim of the present study was to investigate the expression patterns of the markers called cytokeratin 15 (K15), cytokeratin 6 (K6) and monoclonal antibody Ki-67, and also apoptosis in HFs, which can be observed in different parts of the human body. Material and methods. In this study, healthy human HFs were taken by biopsy from 5 various donor sites ofthe human body: the scalp,the leg, the abdomen, the back and waist. HF-containing skin specimens taken using cryosection were stained with hematoxylin & eosin (H&E) and K15, K6, Ki-67 and terminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labelling (TUNEL) immunofluorescence staining protocol was performed. Results. Different skin legions from the human body were examined histologically. While the HFs of scalp tissue showed anatomically obvious hair layers, some hair sections from other regions, like the leg, the abdomen, back and waist, were not as distinct as in the scalp region. According to our findings, K15 expression was highest in the scalp. In addition, the immunoreactivity (IR) intensity of K15 was significantly decreased in the HFs on the waist and abdominal regions, compared to the scalp and back regions (p < 0.001). However, the IR intensity of K6 in the scalp region was statistically significantly higher than the IR intensity of K6 in the abdomen region (p <0.05). Moreover, we showed intraepithelial apoptosis and proliferation of keratinocytes in the bulge of HF. In the study, Ki-67-positive and TUNEL-positive cell numbers were not statistically significant (p > 0.05). Conclusions. Our findings are important for further investigation of molecular aspects of the human hair follicle stem cells compartments in health and disease, which might be a promising model for comparative studies with different human diseases.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Erciyes UniversityErciyes University [EUBAP, TCD-4517]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Unit of Erciyes University (Project number: EUBAP, TCD-4517).en_US
dc.identifier.doi10.17219/acem/74429en_US
dc.identifier.endpage939en_US
dc.identifier.issn1899-5276
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage929en_US
dc.identifier.urihttps://doi.org/10.17219/acem/74429
dc.identifier.urihttps://hdl.handle.net/20.500.12684/2823
dc.identifier.volume27en_US
dc.identifier.wosWOS:000440357400010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWroclaw Medical Univen_US
dc.relation.ispartofAdvances In Clinical And Experimental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmonoclonal antibody Ki-67en_US
dc.subjectterminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labellingen_US
dc.subjecthair follicleen_US
dc.subjectcytokeratin 15en_US
dc.subjectcytokeratin 6en_US
dc.titleAssessment of markers expressed in human hair follicles according to different skin regionsen_US
dc.typeArticleen_US

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