Assessment of markers expressed in human hair follicles according to different skin regions
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wroclaw Medical Univ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background. Body region-dependent hair follicle (HF) characteristics are concerned with follicular size and distribution, and have been demonstrated to have characteristics for each region of the body. Objectives. The aim of the present study was to investigate the expression patterns of the markers called cytokeratin 15 (K15), cytokeratin 6 (K6) and monoclonal antibody Ki-67, and also apoptosis in HFs, which can be observed in different parts of the human body. Material and methods. In this study, healthy human HFs were taken by biopsy from 5 various donor sites ofthe human body: the scalp,the leg, the abdomen, the back and waist. HF-containing skin specimens taken using cryosection were stained with hematoxylin & eosin (H&E) and K15, K6, Ki-67 and terminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labelling (TUNEL) immunofluorescence staining protocol was performed. Results. Different skin legions from the human body were examined histologically. While the HFs of scalp tissue showed anatomically obvious hair layers, some hair sections from other regions, like the leg, the abdomen, back and waist, were not as distinct as in the scalp region. According to our findings, K15 expression was highest in the scalp. In addition, the immunoreactivity (IR) intensity of K15 was significantly decreased in the HFs on the waist and abdominal regions, compared to the scalp and back regions (p < 0.001). However, the IR intensity of K6 in the scalp region was statistically significantly higher than the IR intensity of K6 in the abdomen region (p <0.05). Moreover, we showed intraepithelial apoptosis and proliferation of keratinocytes in the bulge of HF. In the study, Ki-67-positive and TUNEL-positive cell numbers were not statistically significant (p > 0.05). Conclusions. Our findings are important for further investigation of molecular aspects of the human hair follicle stem cells compartments in health and disease, which might be a promising model for comparative studies with different human diseases.
Açıklama
Coruh, Atilla/0000-0002-3592-3433
WOS: 000440357400010
PubMed: 29962115
WOS: 000440357400010
PubMed: 29962115
Anahtar Kelimeler
monoclonal antibody Ki-67, terminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labelling, hair follicle, cytokeratin 15, cytokeratin 6
Kaynak
Advances In Clinical And Experimental Medicine
WoS Q Değeri
Q4
Scopus Q Değeri
Q1
Cilt
27
Sayı
7