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Öğe Baş Dönmesi Olan Hastalarda SCL-90R ve Kısa Formların Kullanımının Değerlendirilmesi(Nobel Ilac, 2014) Ardıç, Fazıl Necdet; Tümkaya, Funda; Tümkaya, Selim; Özdel, Osman; Ankaralı, HandanObjective: Psychiatric symptom screening tests for patients who suffer from imbalance and dizziness are generally used in majority of clinics. Symptom checklist-90 Revised (SCL-90R) is one of these tests. The aims of this study are to analyze the Turkish version of SCL-90R, compare to its short version and investigate whether if it is possible to create a short version or not. Material and Method: The sample of this study consisted of 2100 patients who admitted to tertiary care neurotology department for imbalance and dizziness. Three different SCL-90R versions as the original, Brief Symptom Inventory (BSI) and Brief Symptom checklist-18 (BSI-18) were retrospectively studied. These three tests were evaluated with regards to global severity index, totalpositive symptoms and positive symptom index. The correlation between results were compared by Pearson correlation test. Structural integrity studies were also conducted with using raw data of SCL-90R. Results: There were strong correlations among the three questionnaires. While the structural distribution of questions are consisted of 10 factors in the standard form (SCL-90), the short form (containing 49 questions) which was created from our results was found to have different structural distribution. Moreover the distribution of questions were quite different than the actual structure and the questions from different factors were grouped under different factors. The short form, which was created from our results, showed similar properties with the BSI. The internal consistency reliability test showed significance for 9 of them. Eventually, we created a short form consisting of 9 factors and 49 questions. In this state, it shows similar features with BSI and contains a similar number of questions. Conclusion: Due to the difference in structural analysis, the results obtained by the sub-scales of the questionnaires should be evaluated very carefully. We think the short form we created can be used easily since the general values resulted from it are comparable to the the original questionnaire.Öğe Evaluation of sleep in women with menopause: results of the Pittsburg Sleep Quality Index and polysomnography(Galenos Yayincilik, 2015) Ağan, Kağan; Özmerdivenli, Recep; Değirmenci, Yıldız; Çağlar, Mete; Başbuğ, Alper; Balbay, Ege Güleç; Sungur, Mehmet AliObjective: To investigate subjective sleep quality among women in the menopausal period and to confirm and diagnose the possible sleep disturbances with polysomnographic (PSG) evaluation objectively. Material and Methods: Sixty-seven women with menopause were enrolled in the study. Sociodemographic characteristics and the features of menopause were recorded. We assessed subjective sleep quality with Pittsburg Sleep Quality Index (PSQI). To confirm sleep disturbances and further diagnose the underlying cause, PSG evaluation was performed to women with PSQI scores of >5 who gave their approval. Results: Mean PSQI score of women with normal PSG evaluation was 12.00 +/- 3.16, while it was 11.00 +/- 2.32 in women with abnormal PSG evaluation (p=0.466); 59.7% (n=40) of women had poor sleep quality. Among these, 11 (64.7%) had abnormal results in the PSG evaluation and were diagnosed with obstructive sleep apnea syndrome (OSAS); 54.5% had mild OSAS, 27.3% had moderate, and 18.2% had severe OSAS. Conclusion: PSQI and PSG evaluations would give a chance to demonstrate sleep problems and shed a light on treatment options according to the underlying causes of sleep disturbances in menopause.Öğe Evaluation of Paraoxonase Activity, Total Sialic Acid and Oxidative Stress in Sheep with Ecthyma Contagiosa(Kafkas Univ, Veteriner Fakultesi Dergisi, 2017) Deveci, Hacı Ahmet; Kükürt, Abdulsamed; Uzlu, Erdoğan; Sözdutmaz, İbrahim; Merhan, Oğuz; Aktaş, Sinan; Karapehlivan, MahmutZoonoses are defined by the world health organization as diseases and infections which are transmitted naturally between vertebrate animals and human. Undestanding the zoonotic risk posed by pox viruses in companion animals is important for both human and animal health. contagious ecthyma is highly contagious, zoonotic, viral skin disease that affects sheep, goats and some other domesticated and wild animals. In this present study was detected and evaluated levels of plasma paraoxonase activity (PON1), high-density lipoprotein (HDL), total sialic acid (TSA), malondialdehyde (MDA), nitric oxide (NO) and total blood glutathione (GSH) concentrations in healthy sheep and natural infected sheeps with ecthyma. In healthy sheep, laboratory results were determined as PON1 218.54 +/- 17.93 U/L, TSA 59.89 +/- 5.59 mg/dL, HDL 48.4 +/- 4.88 mg/dL, MDA 8.58 +/- 0.80 mu mol/L, NO 7.78 +/- 1.02 mu mol/L and GSH 21.11 +/- 3.70 mg/dL. These values were found 174.92 +/- 18.68 U/L, 70.1 +/- 6.56 mg/dL, 37.9 +/- 6.47 mg/dL, 11.26 +/- 1.06 mu mol/L, 12.44 +/- 1.90 mu mol/L, 7.79 +/- 0.90 mg/dL respectively in sheeps wich are infected by ecthyma. As a result, it was concluded that there is oxidative stress due to imbalance between pro-oxidant and antioxidant molecules in sheep which are infected by ecthyma, and this imbalance is shaped by increasing oxidant levels.Öğe Evaluation of vitamin B12 level in middle-aged obese women with metabolic and nonmetabolic syndrome: case-control study(Tubitak Scientific & Technical Research Council Turkey, 2012) Baltacı, Davut; Kutlucan, Ali; Öztürk, Serkan; Karabulut, İsmail; Yıldırım, Hayriye Ak; Çeler, Ahmet; Kara, İsmail HamdiAim: To investigate the correlation between vitamin B12 and body mass index (BMI) along with insulin resistance (IR) in middle-aged obese women. Materials and methods: The study was designed as a case-control study. The study group included middle-aged obese women and the control group included aged-matched lean women. Weight, height, and hip and waist circumferences were measured. Biochemical parameters such as fasting and postprandial glucose, vitamin B12 and folic acid levels, and lipid profiles were assayed. Results: Enrolled in the study were 116 middle-aged obese and 103 aged-matched healthy lean women. The vitamin B12 level of the obese women was significantly lower than that of the lean women (244.1 +/- 131.5 pg/mL vs. 336.2 +/- 163.1 pg/mL, P = 0.002). However, there was no significant difference in folic acid levels between the groups (P > 0.05). The vitamin B12 level was similar in the obese women with metabolic syndrome and those without (245.1 +/- 145.3 pg/mL vs. 241.2 +/- 96.5 pg/mL, P > 0.05), but the level in the control group was significantly higher than that of patients with obesity and metabolic syndrome (P = 0.010 and P = 0.020, respectively). Vitamin B12 levels correlated with BMI (r = -0.259, P = 0.003) but not with IR (r = -0.053, P > 0.05). Conclusion: The vitamin B12 concentration was low in obese patients and this level negatively correlated with BMI, but not with homeostasis model assessment-estimated IR (HOMA-IR).Öğe Examining of Published Articles with Respect to Statistical Errors in Medical Sciences(Akad Doktorlar Yayinevi, 2015) Ercan, İlker; Karadeniz, Pınar G.; Cangür, Şengül; Özkaya, Güven; Demirtaş, HakanResearchers who do not have adequate statistical knowledge commit a wide range of critical errors with regard to the design, execution,analysis, presentation and interpretation of their studies. The aim of the present work is to examine the statistical errors of scientific articles. Cross sectional study. Methods: Ninety-five articles published in either Science Citation Index (SCI) or (Science Citation Index-Expanded) SCI-E journals, 122 articles published in non-SCI or non-SCI-E journals were included in this study. The articles were chosen from among those indexed in the PubMed and Bioline databases between the years 2004 and 2010, inclusively. A total of 217 articles had at least one statistical error. The most frequently encountered statistical error was "errors in summarizing data" for articles published in the journals indexed in SCI or SCI-E, as well as non-SCI or non-SCI-E journals. For errors involving "use of an incorrect test" and "statistical symbol errors", there was a statistically significant difference between articles published in journals indexed in SCI or SCI-E and non-SCI or non-SCI-E journals; this difference favored the former. Some action should be taken by researchers and editors to prevent the introduction of statistical errors into scientific publications. Researchers (i) should have a basic statistical knowledge, (ii) should consult a biostatistician at the planning, analyzing, interpreting and reporting stages of a study. Furthermore, editors should send studies that have been submitted to their journal to a biostatistician during the review process.Öğe Evaluation of the functional status of patients with chronic obstructive pulmonary disease via the modeling approach(Addis Ababa Univ, Dept Community Health, 2019) Cangür, Şengül; Balbay, Ege Güleç; Ankaralı, Handan; Berik, SinemBackground: Chronic obstructive pulmonary disease (COPD) is common worldwide. COPD has negative effects on the quality of life of the patient due to restrictions on daily activities. It can cause a major healthcare burden according to the stage and grade of disease. Objective: The purpose of this study was to determine the physical and psychological functional capacity factors affecting patients' functional performance and to develop a COPD-specific functional status model using these factors. Methods: A group of randomly selected patients (n=183) diagnosed with COPD at a university hospital in Turkey comprised the study sample. Physical examinations were carried out on all patients and they completed questionnaires that included socio demographic and disease characteristics, the Hospital Anxiety and Depression Scale, Functional Performance Inventory and Short Form-36 Survey. The proposed theoretical model was analyzed in the LISREL program using the structural equation modeling approach. Results: We found that the independent latent variable of the physiological functional capacity yielded a significant positive effect on the dependent latent variable of functional performance, while the psychological functional capacity produced no significant effect. Conclusions: The COPD-specific functional status model should prove to be an effective and beneficial tool for issues such as treatment maintenance and for the preservation or improvement of patients' quality of life.Öğe Evaluation of Elderly Patients at Check-Up Polyclinics for Anemia, Serum Folate and Cobalamin Levels(Walter De Gruyter Gmbh, 2010) Kara, İsmail Hamdi; Kandiş, Hayati; Bahçebaşı, Talat; Köylü, Oya Kıreker; Sayın, Sultan; Demirin, Hilmi; Memişoğulları, RamazanIntroduction: It was aimed to evaluate CBC analyses (HCT, MCV, MCH, MCHC, RDW and PLT), folic acid, cobalamin, serum iron and serum iron binding capacity and make a comparison according to gender in patients >50 years applied to check-up outpatients' clinic. Material and Method: 20 male and 35 female, total 55, consecutive patients who have median age 59.0 (50-85) yrs, applied to check-up polyclinic were included. Result: While median B12 vitamin and folic acid levels in men as follows: 170 (1501000) pg/mL and 6.4 (6.3-7.7) ng/mL; these values for women as follows: 261 (1501000) pg/mL and 9.3 (1.8-17.7) ng/mL (P<0.01 and P<0.01), consecutively. Cobalamin deficiency is common in both of gender. It was determined that cobalamin deficiency in male was 70%, and in female, 45.7%, (P=0.07), consecutively. Folic acid level is found in only one female case as <2 ng/mL. TSI% is lower in female (in male as 26.6% vs. in female as 18.1%; P<0.0001). MCV in eight (14.5%) cases was below 80 fL and MCV in nine (16.4%) cases was above 90 fL; however, MCV in none of cases was above 100 fL. Conclusion: In periodical examination of elderly, a high level of cobalamin deficiency could be determined; also the prevalence of cobalamin deficiency could be changed with accepted cut-off level. Present study showed it is important to consider cobalamin and iron deficiency in periodical examination of elderly.Öğe Evaluation of Bone Mineral Density and Bone Turnover Markers in Patients with non-Cirrhotic Chronic Hepatitis B(Ortadogu Ad Pres & Publ Co, 2012) Çelebi, Güven; Sarıkaya, Selda; Özdolap, Şenay; Mungan, Görkem; Ankaralı, Handan; Çelik, Yavuz; Akduman, DenizObjective: To evaluate the bone mineral density (BMD) and the biochemical markers of bone turnover in non-cirrhotic chronic hepatitis B patients. Material and Methods: We compared 18-65 years old non-cirrhotic chronic hepatitis B patients and healthy controls for BMD and biochemical markers of bone turnover. BMD was measured at the lumbar spine and the left hip using DXA. Results: There were 31 hepatitis B patients (19 males, 12 females) and 72 healthy controls (56 males, 16 females) in the study. Their mean age was 37.4 +/- 10.0 years in the study group and 38.5 +/- 7.1 years in the controls. There were no differences between the two groups for the mean BMD levels and T scores. However, left hip BMD and T scores were significantly lower in male patients with chronic hepatitis B infection when compared to males in the control group. Serum calcium, phosphate, osteocalcine, intact parathyroid hormone, deoxypyridinoline and 25-hydroxycholecalciferol levels did not differ between the groups. Conclusion: In our study, left hip BMD and T scores were found to be significantly lower in the male patients with chronic hepatitis B infection when compared to males in the control group, however a significant difference was not detected when all cases were compared with the control group. Therefore, we suppose that further studies on a larger population are needed in this issue.Öğe Evaluating clonidine response in children and adolescents with attention-deficit/hyperactivity disorder(Amer Inst Mathematical Sciences-Aims, 2018) Kütük, Meryem Özlem; Güler, Gülen; Tufan, Ali Evren; Sungur, Mehmet Ali; Topal, Zehra; Kütük, ÖzgürAttention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in childhood, which is generally treated with stimulant and non-stimulant medications. However, 10-30% of patients in clinical setting do not present with adequate response to initial stimulant treatment. Thereby, clonidine may be considered for those patients who have failed to respond to psychostimulant/atomoxetine monotherapy or as an augmentation for inadequate response/comorbidity. This observational study evaluated its effectiveness as a single drug in ADHD cases unresponsive to previous treatment trials. Seventeen ADHD cases that were non-responders to stimulant, non-stimulant and combination therapy for the primary symptoms of ADHD were included in the study. Four cases dropped out before follow up, leaving thirteen cases who were administered immediate release clonidine treatment alone with a mean dose of 0.2 +/- 0.05 mg/day at baseline. The trial lasted for 12 weeks, and treatment outcomes were evaluated by the Turgay DSM-IV Based Child and Adolescent Behavior Disorders Screening and Rating Scale (T-DSM-IV-S) and the Clinical Global Impressions-Severity (CGI-S) and Improvement (CGI-I) scales. Mean age of the sample was 12.5 years (SD = 3.0) and eleven of the subjects had another comorbid psychopathology. Only two cases were evaluated as "very much improved", while another patient was judged to be "minimally improved" after 12 weeks of clonidine treatment. Attrition during follow-up was associated with higher median scores on the hyperactivity and impulsivity subscales (Mann-Whitney U test, p = 0.02). According to the T-DSM-IV-S, CGI-S, and CGI-I scales, clonidine treatment by itself had minimal benefits in this sample of treatment of refractory cases with ADHD evaluated at the study center. Clonidine is not available in Turkey pharmaceutical marketing system and patients' access to drug is limited. Our results provide first data regarding the use of clonidine in Turkish ADHD patients.Öğe Evaluating antimicrobial and antioxidant capacity of endemic Phlomis russeliana from Turkey and its antiproliferative effect on Human Caco-2 Cell Lines(Acad Brasileira De Ciencias, 2019) Alpay, Merve; Dülger, Görkem; Şahin, İbrahim E.; Dülger, BaşaranIn this study, the antimicrobial, antioxidant and antitumor activity of ethanol extracts obtained from Phlomis russeliana (Sims.) Lag. ex Benth. (Lamiaceae) were evaluated. Disc diffusion and microdilution methods were used to test the extracts for antimicrobial activity against seven bacteria strains (Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445 and Pseudomonas aeruginosa ATCC 27853) and four yeast strains (Kluyveromyces fragilis ATCC 8608, Rhodotorula rubra ATCC 70403, Debaryomyces hansenii DSM 70238 and Candida albicans ATCC 10239). Notably, they were more effective against the yeast strains than the bacterial strains. Of the yeast cultures, D. hanseii was among the most susceptible, having an inhibition zone of 16.2 mm with minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of 64(128)mu g/ml, respectively. For cytotoxic determination, Caco-2 cells were cultured as per ATCC protocol, and were treated with log concentrations (5-80 mg/ml) of P. russeliana. The potency of cell growth inhibition for each extract was expressed as an IC50 value. Moreover, oxidant capacity was evaluated via TOC assay. This product induced antiproliferative activity of 31.33% at 40 mg/nil and 20.96% at 80 mg/ml, without toxic effects on cells, although the oxidant capacity was decreased to 27.06 +/- 0.7 nm in the 80 mg/nilapplied group compared to 47.9 +/- 1.8 mn in the untreated one. Advanced pharmacological studies are needed to further evaluate P. russeliana for distinctive features.Öğe Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA External Quality Assessment National Program Results(Ankara Microbiology Soc, 2018) Karataylı, Ersin; Soydemir, Ege; Aksoy, Zeynep Büşra; Kızılpınar, Mehtap; Koçak, Aylin Altay; Karataylı, Senem Ceren; Toraman, Zülal AşçıMOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load. The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.Öğe GSM-like radiofrequency exposure induces apoptosis via caspase-dependent pathway in infant rabbits(Comenius Univ, 2016) Meral, Öğünç; Özgür, Elçin; Kısmalı, Görkem; Güler, Gülnur; Alpay, Merve; Sel, Tevhide; Seyhan, NesrinBACKGROUND: There have been several Radio Frequency (RF) field researches on various populations and groups of different ages in recent years. However, the most important group for research has been declared as the pregnant women and their babies. OBJECTIVE: The aim of the study was to analyse the effect on apoptotic factors of RF fields on newborn rabbit liver tissues. MATERIALS AND METHODS: Cytochrome c and AIF (Apoptosis Inducing Factor) levels were measured by western blot and caspase 1, 3 and 9 activities were measured by colorimetric method. RESULTS: Cytochrome c and AIF levels were not altered, but all caspase activities were increased in female infant rabbits that exposed to 1800 MHz GSM-like RF signals when they reached 1 month of age and caspase 1 and caspase 3 levels were decreased in male infant rabbits that exposed to 1800 MHz GSM-like RF signals between 15th and 22nd days of the gestational period. Results showed that 1800 MHz GSM-like RF exposure might lead to apoptosis in infant rabbit's liver tissues. CONCLUSION: According to the results, we suggest that postnatal RF exposure causes caspase dependent apoptosis in female infant rabbits liver tissues (Tab. 1, Fig. 2, Ref. 27). Text in PDF www.elis.sk.Öğe High serum homocysteine levels correlate with a decrease in the blood flow velocity of the ophthalmic artery in highway toll collectors(Tohoku Univ Medical Press, 2007) Memişoğulları, Ramazan; Yüksel, Harun; Coşkun, Abdurrahman; Yüksel, Hatice Kurt; Yazgan, Ömer; Bilgin, CahitHighway workers, such as policemen, automotive service companies, and toll collectors, are placed at risk of the accelerated atherosclerotic process, since recent studies have suggested that exposure to exhaust particles and ambient air pollution increases carotid intima-media thickness and reduces ocular blood flow velocity. Therefore, we assessed the relationship between serum homocysteine, a potential parameter for atherosclerosis, and the ocular blood flow velocity and the resistivity index in highway toll collectors. The peak systolic and end diastolic flow velocities and the resistivity index were measured in 22 toll collectors and 24 control subjects by color Doppler ultrasonography. The resistivity index, which is an indirect measure of the atherosclerotic process, was calculated: resistivity index = (peak systolic velocity - end diastolic velocity)/peak systolic velocity. Serum homocysteine levels were determined by fluorometric high-performance liquid chromatography. In the highway toll collectors, the serum homocysteine level (14.4 +/- 4.8 mu mol/l; p < 0.005) and the resistivity index of the ophthalmic artery (0.741 +/- 0.015; p < 0.05) were higher and the ophthalmic blood flow velocity (33.0 < 3.0 cm/s; p < 0.001) was lower than those in the controls (10.6 +/- 3.1,mu mol/l; 0.728 +/- 0.023; 36.8 +/- 2.2 cm/s; respectively). There were significant correlations between the serum homocysteine level and ophthalmic artery resistivity index in both highway toll collectors (p < 0.001) and controls (p < 0.005). Exposure to exhaust particles might increase the serum homocysteine level, which in turn could lead to the decreased ocular blood flow and the increased resistivity index.Öğe High ionic strength depresses muscle contractility by decreasing both force per cross-bridge and the number of strongly attached cross-bridges(Springer, 2015) Wang, Li; Bahadır, Anzel; Kawai, MasatakaAn increase in ionic strength (IS) lowers Ca2+ activated tension in muscle fibres, however, its molecular mechanism is not well understood. In this study, we used single rabbit psoas fibres to perform sinusoidal analyses. During Ca2+ activation, the effects of ligands (ATP, Pi, and ADP) at IS ranging 150-300 mM were studied on three rate constants to characterize elementary steps of the cross-bridge cycle. The IS effects were studied because a change in IS modifies the inter- and intra-molecular interactions, hence they may shed light on the molecular mechanisms of force generation. Both the ATP binding affinity (K (1)) and the ADP binding affinity (K (0)) increased to 2-3x, and the Pi binding affinity (K (5)) decreased to 1/2, when IS was raised from 150 to 300 mM. The effect on ATP/ADP can be explained by stereospecific and hydrophobic interaction, and the effect on Pi can be explained by the electrostatic interaction with myosin. The increase in IS increased cross-bridge detachment steps (k (2) and k (-4)), indicating that electrostatic repulsion promotes these steps. However, IS did not affect attachment steps (k (-2) and k (4)). Consequently, the equilibrium constant of the detachment step (K (2)) increased by similar to 100 %, and the force generation step (K (4)) decreased by similar to 30 %. These effects together diminished the number of force-generating cross-bridges by 11 %. Force/cross-bridge (T (56)) decreased by 26 %, which correlates well with a decrease in the Debye length that limits the ionic atmosphere where ionic interactions take place. We conclude that the major effect of IS is a decrease in force/cross-bridge, but a decrease in the number of force generating cross-bridge also takes place. The stiffness during rigor induction did not change with IS, demonstrating that in-series compliance is not much affected by IS.Öğe HBSAG, ANTI-HCV, ANTI-HIV 1/2, AND SYPHILIS SEROPREVALENCE IN BLOOD DONORS IN EASTERN MARMARA REGION, TURKEY AND AN OVERVIEW OF TRANSFUSION TRANSMITTED INFECTIONS IN TURKEY(Carbone Editore, 2016) Altındiş, Mustafa; Köroğlu, Mehmet; Mutlu, Birsen; Demiray, Tayfur; Dal, Tuba; Şahin, İdris; Özçelik, ÜmitIntroduction: Blood transfusion and component therapies are life-saving medical practices. However these therapies, are not without risks and may lead to the transmission of infectious agents from donor to recipient. Screening of blood donations for infectious agents is necessary to achieve transfusion safety. The aim of the study was to determine the six-years seroprevalence of HBV (hepatitis B virus), HCV (hepatitis C virus), HIV (human immunodeficiency virus), and syphilis in blood donors and to present an overview of transfusion transmitted infections in Turkey. Material and methods: A total of 150,787 volunteer blood donors who admitted to blood centers located in Eastern Marmara Region, Turkey between January 2009 and October 2014 were included in this study. HBsAg (hepatitis B surface antigen), anti-HCV (hepatitis C virus antibody), and HIV-1/2 antibody were detected by using Chemiluminescent Microparticle Enzyme Immunoassay (CMIA) method.. Syphilis screening was performed by using VDRL (Venereal Disease Research Laboratory) test in the first three years and by CMIA-based treponemal test in the last three years. Results: Among the blood donors, 140,536 (93.2%) were male and 10,251 (6.8%) female. Out of all donors, 2,019 (1.33%) donors were positive for at least one screening test. The seroprevalence of HBV, HCV, HIV, and syphilis were 0.8 % (n=1340), 0.38% (n=578), 0,0025% (n=38) and 0,004 % (n=63), respectively. A significant decrease was observed in HBV seroprevalence by years (p <0.005) Conclusion: The risk of transfusion-transmitted infections can be decreased by effectively using questionnaires and screening tests in blood donation centers, HBV vaccination programs, and awareness-raising activities. It is necessary to use improved screening methods for detection of transfusion-transmitted infection agents.Öğe Heavy Metal Biosorption and Plasmid Profiles of Heavy Metal Resistance Bacteria isolated from Soil Samples of Thermal power, Canakkale, Turkey(Research Journal Biotechnology, 2017) Dülger, Görkem; Akı, CüneytThe aim of this study is to investigate the isolation, characterization, determination of plasmid contents and metal absorption capacities of heavy metal-resistant bacteria from soil samples of thermal power. Samples were collected from soil in the area of thermal power. To define the resistance of heavy metal, metal solutions of ZnCl2, FeCl3 6 center dot H2O, Pb(NO3)(2) and CuSO4 in varying concentrations were added to culture media by using MIC method. The bacteria of highest MIC level were defined through VITEK 2 system and their plasmid profiles were determined. The metal biosorption capacities of two isolates were defined through ICP-AES. In total, 37 heavy metal resistant bacteria were isolated from soil samples. Results showed that 15 of them contained plasmid DNA. Finally, biosorption experiments with two isolates with the highest metal resistance were performed. While the metal having the highest growing speed of biosorption among Fe, Zn, Pb and Cu metals which were used in this study for Bacillus mycoides (isolate 26) is Zn, it is followed by Pb, Fe and Cu orderly. While the metal having the highest growing speed of biosorption for Enterobacter cloacea complex (isolate 10) is Pb, it is followed by Zn, Fe and Cu orderly.Öğe Helcococcus kunzii Detected from the Wound Site After Bone Fracture: A Rare Agent(Bilimsel Tip Yayinevi, 2019) Dönmez, Betül; Çalışkan, Emel; Arıcan, MehmetHelcococcus kunzii is a gram-positive and catalase negative cocci that can be pathogenic especially in lower extremity wounds and cellulite of diabetic patients. This study aimed to report the case of a diabetic patient with an advanced wound infection after bone fracture caused by H. kunzii with Staphylococcus aureus. Beta-haemolytic staphylococci-like colonies and alpha-haemolytic streptococci-like colonies were observed in the wound sample. Catalase and oxidase tests of alpha haemolytic colonies were found negative. In the identification with VITEK 2 (bioMerieux, France), the bacteria were identified as S. aureus and H. kunzii. H. kunzii isolate was susceptible to ampicillin, ceftriaxone, cefepime, cefotaxime and vancomycin; resistant to erythromycin and clindamycin. Amoxicillin-clavulanic acid was used for the treatment of the patient, and the patient recovered. This case supports the knowledge that H. kunzii can cause soft tissue infection with bacteria such as S. aureus.Öğe Genetic origin of Behcet's disease population in Denizli, Turkey; population genetics data analysis; historical demography and geographical perspectives based on beta-globin gene cluster haplotype variation(Nature Publishing Group, 2017) Öztürk, Onur; Arıkan, Sanem; Bahadır, Anzel; Atalay, Ayfer; Atalay, Erol Ö.In our study, we aimed to investigate the possible genetic drift, relationships, expansion and historical origin based on haplotype frequencies of the beta-globin gene cluster of normal and Behcet's disease (BD) population in Denizli, Turkey. We examined blood DNA samples obtained from our DNA bank. The association of population genetic parameters such as haplotypes, diversity, differentiation, Hardy Weinberg equilibrium and demographic analysis for two populations was performed by Arlequin ver. 3.5. Our results show that both populations have high similarity in genetic parameters in terms of development and expansion based on haplotype diversity through the history. We found that historical levels of gene flow were significantly higher between the two populations. According to historical population, growth parameter of tau values for normal and BD populations dated approximately 42 000 to 38 000 ybp, respectively. In conclusion, historically, two populations show similar genetic parameters and unimodal growth distribution. Our results are consistent with the view that the BD may have occurred in area, independent from Silk Road.Öğe Gender Specificity of Genistein Treatment in Penicillin-Induced Epileptiform Activity in Rats(Springer, 2016) Bahadır, Anzel; Demir, Şerif; Orallar, Hayriye; Beyazçiçek, Ersin; Çetinkaya, Ayhan; Ankaralı, Seyit; Ankaralı, HandanWe investigated gender-dependent differences of genistein (isoflavone phytoestrogen) treatment in a penicillin-induced experimental epilepsy rat model. Twenty-eight adult Wistar Albino rats (14 females and 14 males) were devided into four groups, control and genistein-treatmed males and females. Genistein (100 mu g/kg, i.p) or saline was given during 15 days before the electrocorticography (ECoG) recordings. The epileptiform activity was induced by penicillin G potassium salt (500 IU) injections into the left somatomotor cortex. Significant differences among the groups were found in the latency to onset of epileptiform activity. This value in the female control group was significantly longer than the latencies in the male control, male genistein, and female genistein groups (respectively, P = 0.002, 0.015, and 0.032). There were no significant differences regarding the spike/wave frequencies and amplitudes in epileptiform activity between female/male genistein and control groups within all observation intervals (P > 0.05). Thus, genistein exerts a proconvulsant effect in the penicillin-induced epilepsy model, and the effect demonstrates the clear gender specificity related to the specificity of hormonal backgrounds in males and females.Öğe Further Evidence for the Role of Nitric Oxide in Maternal Aggression: Effects of L-NAME on Maternal Aggression towards Female Intruders in Wistar Rats(Acad Sciences Czech Republic, Inst Physiology, 2009) Ankaralı, Seyit; Ankaralı, Handan; Marangoz, CaferIt has been shown that nitric oxide (NO) increases aggression in male mice, whereas it decreases aggression in lactating female mice and prairie voles. It is also known that aggression can be exhibited at different levels in rodent species, strain or subtypes. The aims of this study were to investigate the proportion of aggressiveness in Wistar rats, the effect of intraperitoneally administered nonspecific nitric oxide synthase (NOS) inhibitor L-NAME (N-G-nitro L-arginine methyl ester) on maternal aggression towards female intruders, and whether these effects are due to NO production or not. Rats were given saline intraperitoneally on the postpartum Day 2 and aggression levels were recorded. The same rats were given 60 mg/kg L-NAME or D-NAME (N-G-nitro D-arginine methyl ester) on the postpartum Day 3 and their effects on aggression levels were compared to saline. While L-NAME administration did not cause any differences in the total number of aggressive behavior, aggression duration and aggression intensity, it reduced the proportion of animals showing aggressive behavior. In addition, the latency of the first aggression was significantly increased by L-NAME. In the D-NAME group, however, no significant change was found. Our results have shown that L-NAME reduces maternal aggression towards female intruders in Wistar rats through inhibition of NO production. These results suggest that the role of NO in offensive and defensive maternal aggression shares neural mechanisms.
