Erythropoietin stimulates patellar tendon healing in rats
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Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Backround: Erythropoietin (EPO), regulating erythropoiesis, is used to provide protective and regenerative activity in non-haematopoietic tissues. There is insufficient knowledge about the role of EPO activity in tendon healing. Therefore, we investigated the effect of EPO treatment on healing in rat patellar tendons. Methods: One hundred and twenty-six, four-month-old male Sprague-Dawley rats were randomly assigned to three experimental groups: 1, no treatment; 2, treatment with isotonic saline (NaCl) and 3, treatment with EPO. Each group was randomly subdivided into two groups for sacrifice at three (1a, 2a, 3a) or six weeks (1b, 2b, 3b). Complete incision of the left patellar tendon from the distal patellar pole was performed. We applied body casts for 20 days after the incised edges of the patellar tendon were brought together with a surgical technique. Both legs were harvested and specimens from each group underwent histological, biomechanical, and protein mRNA expression analyses. Results: There were statistically significant differences in the ultimate breaking force between the EPO group and others at both weeks three and six (p<0.05); significant differences in fibroblast proliferation, capillary vessel formation, and local inflammation were found between groups 1 a and 3a, and 2a and 3a (p<0.05). There were statistical differences between la, 3a and 2a, 3a for Col III, TGF-beta 1, and VEGF and between 1b, 3b and 2b, 3b for Col I, Col III, TGF-beta 1, and VEGF mRNA expressions. Conclusion: EPO had an additive effect with surgery on the injured tendon healing process in rats compared to the control groups biomechanically, histopathologically and with tissue protein mRNA expression. (C) 2015 Elsevier B.V. All rights reserved.
Açıklama
Yaykasli, Kursat/0000-0001-7550-6370; Kandis, Hayati/0000-0001-9151-6050; Kaya, Ertugrul/0000-0003-0081-682X
WOS: 000367634100003
PubMed: 26067853
WOS: 000367634100003
PubMed: 26067853
Anahtar Kelimeler
Rat, Erythropoietin, Tendon healing, Patellar tendon injury
Kaynak
Knee
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
22
Sayı
6
