Antileukemic effects of piperlongumine and alpha lipoic acid combination on Jurkat, MEC1 and NB4 cells in vitro
Yükleniyor...
Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Medknow Publications & Media Pvt Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim of Study: This research indicated to evaluate the effects of piperlongumine (PL), a biologically active alkaloid, and alpha lipoic acid (ALA), a naturally occurring cofactor existed in multienzyme complexes regulating metabolism on leukemia cells. Excessive production of reactive oxygen species (ROS) can lead to oxidative stress, a state that has been observed in several hematopoietic malignancies, including acute and chronic myeloid leukemias. The importance of the association between oxidative stress and malignancy is not currently clear; however, there is evidence that tumor.derived ROS may promote cell survival, migration and metastasis, proliferation and even drug.resistance depending on the origin of the cancer. Increased oxidative stress in leukemic cells may represent a potential therapeutic target, although there are differing opinions on whether therapeutic strategies should aim to antagonize or further promote oxidative stress in leukemic cells. Materials and Methods: The effects of PL alone (5, 15, 30 M) and in combination (30 M) with ALA (200 M) on Jurkat, NB4 and MEC1 leukemia cell lines were investigated through MTT, caspase-3 and cyclooxygenase-2 (COX-2) activities. Results: Inhibition of COX-2 and the induction of caspase.3 cleavage in Nb4 (acute promyelocytic leukemia) cells were found to be significant following PL application and synergistic effects with combination of ALA (inhibition of COX-2 as 23.74% and 3.55-fold increase of caspase-3). Conclusion: PL and ALA may have a potential value as a therapeutic agent for patients with acute promyelocytic leukemia.
Açıklama
WOS: 000382405800020
PubMed: 27461609
PubMed: 27461609
Anahtar Kelimeler
Alpha lipoic acid, COX-2, leukemic cell lines, oxidative stress, piperlongumine
Kaynak
Journal Of Cancer Research And Therapeutics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
12
Sayı
2
