Protective effect of heparin in the end organ ischemia/reperfusion injury of the lungs and heart

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dc.contributor.authorGedik, Hikmet Selçuk
dc.contributor.authorKorkmaz, Kemal
dc.contributor.authorErdem, Havva
dc.contributor.authorKarakılıç, Evvah
dc.contributor.authorLafçı, Gökhan
dc.contributor.authorAnkaralı, Handan
dc.date.accessioned2020-04-30T23:21:51Z
dc.date.available2020-04-30T23:21:51Z
dc.date.issued2012
dc.departmentDÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.descriptionAnkarali, Handan Camdeviren/0000-0002-3613-0523en_US
dc.descriptionWOS: 000315283500001en_US
dc.descriptionPubMed: 23151309en_US
dc.description.abstractBackground: Ischemia/reperfusion (I/R) injury is harmful to the cardiovascular system and is responsible for the inflammatory response and multiple organ dysfunctions. In this study we investigated the effect of activated clotting time level on the aortic cross-clamping triggers a systemic inflammatory response and it effects to lungs and heart. Methods: End organ concentrations of interleukin-6 (IL-6), myeloperoxidase (MPO) and heat shock protein 70 (HSP-70) were determined in four groups of Spraque Dawley rats: ischemic control (operation with cross clamping received IP of 0.9% saline at 2 ml/kg n=7) Sham (operation without cross clamping, n=7), heparin (ACT level about 200), High dose heparin (ACT level up to 600) The infrarenal aorta was clamped for 45 minutes by a mini cross clamp approximately 1cm below the renal artery and 1cm iliac bifurcation in all groups without sham group. Heparin was given intraperitoneal (IP) before the procedure. All rats were sacrificed 48 h later. In a second experiment, the effects of I/R on remote organs (lungs and heart) were harvested for analysis. We evaluated tissue levels of myeloperoxidase, interleukin-6, and heat shock protein (HSP-70) were analyzed as markers oxidative stress and inflammation. Histological analyses of the organs were performed. Results: The lungs paranchymal MPO and HSP-70 levels significantly decreased (p<0.05), but IL-6 level was not significant (p>0.05) in heparinized and high dose heparinized groups when compared to ischemic control group. Histopathological evaluation as edema, cell degeneration, inflammation statistically significantly decreased in both group heparinized and high dose heparinized compared with ischemic control group (p<0.05). The heart paranchymal MPO levels significantly decreased in heparinized and high dose heparinized groups when compared to ischemic control group (p=0.023). IL-6, HSP-70 levels were not significant heparinized and high dose heparinized groups when compared to ischemic control group (p=0.0489, p=0.0143). Histopathological evaluation as degeneration statistically significantly decreased in both group heparinized and High dose heparinized compared with ischemic control group (p=0.005). Conclusion: Heparin decreased remote organs injury on the lung and heart after ischemia/reperfusion of infra-renal section of the body in the rat model. So, we should be balance to act level for avoid to I/R injury per operative and early post operative period as providing ACT level nearly 200.en_US
dc.identifier.doi10.1186/1749-8090-7-123en_US
dc.identifier.issn1749-8090
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1186/1749-8090-7-123
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4260
dc.identifier.volume7en_US
dc.identifier.wosWOS:000315283500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofJournal Of Cardiothoracic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleProtective effect of heparin in the end organ ischemia/reperfusion injury of the lungs and hearten_US
dc.typeArticleen_US

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