The investigation of the molecular changes during lipopolysaccharide-induced systemic inflammation on rat hippocampus by using FTIR spectroscopy
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study is to reveal the molecular changes accompanying the neuronal hyper-excitability during lipopolysaccharide (LPS)-induced systemic inflammation on rat hippocampus using Fourier transform infrared (FTIR) spectroscopy. For this aim, the body temperature of Wistar albino rats administered LPS or saline was recorded by radiotelemetry. The animals were decapitated when their body temperature began to decrease by 0.5 degrees C after LPS treatment and the hippocampi of them were examined by FTIR spectroscopy. The results indicated that systemic inflammation caused lipid peroxidation, an increase in the amounts of lipids, proteins and nucleic acids, a decrease in membrane order, an increase in membrane dynamics and changes in the secondary structure of proteins. Principal component analysis successfully separated control and LPS-treated groups. In conclusion, significant structural, compositional and functional alterations occur in the hippocampus during systemic inflammation and these changes may have specific characteristics which can lead to neuronal hyper-excitability.
Açıklama
Anahtar Kelimeler
Fourier transform infrared (FTIR) spectroscopy, hippocampus, lipid structure, lipopolysaccharide (LPS), neuronal excitability, protein structure, systemic inflammation, Transform Infrared-Spectroscopy, Thiol Redox State, Mitochondrial Dysfunction, Seizure Susceptibility, Ir Microspectroscopy, Hypothermic Response, Ionizing-Radiation, Protein Oxidation, Nitric-Oxide, Human-Cells
Kaynak
Journal of Biophotonics
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
17
Sayı
6
