Investigation of plasmid mediated AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae isolates by phenotypic and genotypic

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dc.contributor.authorÇalışkan, Emel
dc.contributor.authorCoşkun, Umut Safiye Say
dc.contributor.authorDülger, Görkem
dc.contributor.authorKılınçel, Özge
dc.contributor.authorAnkaralı, Handan
dc.contributor.authorŞahin, İdris
dc.date.accessioned2020-04-30T23:18:39Z
dc.date.available2020-04-30T23:18:39Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000471264700012en_US
dc.descriptionPubMed: 31189291en_US
dc.description.abstractObjective: To investigate the susceptibility and specificity of the phenotypic methods to determine plasmid-mediated AmpC. Methods: The cross-sectional study was conducted at Duzce University Faculty of Medicine, Microbiology Laboratory from January 2015 to June 2016, and comprised Escherichia coli and Klebsiella pneumonia isolates intermediate susceptible or resistant to cefoxitine. Combined disk diffusion test, double disc synergy test, agar gradient test and polymerase chain reaction were used to detect plasmid-mediated AmpC. Results: Of the 2024 E. coli samples, 44(2.17%), and of the 792 K. pneumoniae samples, 16(2%) were included. Combined disk diffusion test had susceptibility of 68% and specificity of 50%; double disc synergy test 24% and 82%; and agar gradient test 40% and 68%. Of the isolates positively detected by polymerase chain reaction method, more than one gene region positivity was detected in 15(25%) isolates. Conclusion: All three phenotypic methods were found to be insufficient to detect plasmid-mediated AmpC positivity.en_US
dc.description.sponsorshipResearch Fund of Duzce UniversityDuzce Universityen_US
dc.description.sponsorshipThe Research Fund of Duzce University.en_US
dc.identifier.endpage839en_US
dc.identifier.issn0030-9982
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage834en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3459
dc.identifier.volume69en_US
dc.identifier.wosWOS:000471264700012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPakistan Medical Assocen_US
dc.relation.ispartofJournal Of The Pakistan Medical Associationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlasmid-mediated AmpC beta-lactamasesen_US
dc.subjectPCRen_US
dc.subjectEscherichia colien_US
dc.subjectKlebsiella pneumoniaeen_US
dc.titleInvestigation of plasmid mediated AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae isolates by phenotypic and genotypicen_US
dc.typeArticleen_US

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