Investigation of the Effects of Probiotic Bacteria on Bacterial Translocation that Developed During Diagnostic Laparoscopy: An Experimental Study

Basit Öğe Kaydı
dc.contributor.authorŞahin, İdris
dc.contributor.authorAcar, Selda
dc.contributor.authorÖzaydın, İsmet
dc.contributor.authorÖzaydın, Çiğdem
dc.contributor.authorÇalışkan, Emel
dc.contributor.authorYavuz, Mehmet Tevfik
dc.contributor.authorİskender, Abdülkadir
dc.date.accessioned2020-04-30T23:18:41Z
dc.date.available2020-04-30T23:18:41Z
dc.date.issued2012
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000311330900014en_US
dc.descriptionPubMed: 23188579en_US
dc.description.abstractProbiotics which are non-pathogenic live microorganisms ingested along with food or as dietary supplements, are thought to be beneficial to the host by supporting the microbial balance in digestive system. Various studies suggest that the effects of probiotics on the intestinal mucosa and immunity are protective against bacterial translocation. We aimed to investigate bacterial translocation related to the amount of CO2 insufflation given during laparoscopy and the effect of probiotic bacteria in an experimental peritonitis model. In this study 60 Wistar rats were used in six groups consisting of 10 rats. Group 1, 3 and 5 consisted of the rats that were fed without probiotics, while the rats in Group 2, 4, and 6 were fed with water containing 5 x 10(8) cfu/ml probiotic bacteria complex (Bifidobacterium lactis, Lactobacillus bulgaricus, Streptococcus thermophilus) for 15 days. To generate experimental peritonitis, 2 x 10(7) cfu/ml Escherichia coli ATCC 25922 was inoculated intraperitoneally to all of the rats. Thereafter, laparoscopy was applied in all groups. Application in Group 1 and Group 2 was without CO2; Group 3 and Group 4 with 14 mmHg CO2 insufflation, and Group 5 and Group 6 with 20 mmHg CO2 insufflation. Blood samples were taken in 2nd, 4th, and 6th hours. Mesenteric lymph node, liver and spleen samples were taken at 6th hour when the rats were sacrified and then these were evaluated microbiologically with qualitative and quantitative methods. Bacterial translocation and bacteremia were found in the rats that were undergone experimental peritonitis during laparoscopy. All positive tissue and blood cultures yielded E.coli. The highest level of bacterial translocation was found to be in mesenteric lymph nodes (in 3/10, 6/10 and 10/10 in groups 1, 3 and 5 fed without probiotics, respectively; in 2/10, 3/10 and 4/10 in groups 2, 4 and 6 fed with probiotics, respectively). The bacterial translocation rates were found to be related to the increased CO2 insufflation. It was found that probiotic bacteria were more effective for decreasing bacterial translocation rates and bacteremia in the groups that were given high CO2 pressure during laparoscopy. It was also found that these results were correlated with bacterial translocation per gram of tissue. As an example, the quantitative bacterial growth values detected in mesenteric lymph node were 5.4 +/- 2.9 x 10(3), 10.6 +/- 3.3 x 10(3) and 21.5 +/- 12.4 x 10(3) cfu/g in groups 1, 3 and 5, fed without probiotics, respectively; and 2.0 +/- 1.3 x 10(3), 3.8 +/- 1.9 x 10(3) and 9.0 +/- 3.1 x 10(3) cfu/g in groups 2, 4 and 6, fed with probiotics, respectively. Our data emphasized that probiotic bacteria may be used as prophylactic agents for the prevention of bacterial translocation during laparoscopy, however comprehensive and clinical studies are needed to support these experimental results.en_US
dc.identifier.endpage670en_US
dc.identifier.issn0374-9096
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage660en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3479
dc.identifier.volume46en_US
dc.identifier.wosWOS:000311330900014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacterial translocationen_US
dc.subjectperitonitisen_US
dc.subjectpneumoperitoneumen_US
dc.subjectprobiotic bacteriaen_US
dc.titleInvestigation of the Effects of Probiotic Bacteria on Bacterial Translocation that Developed During Diagnostic Laparoscopy: An Experimental Studyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu