Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects

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dc.contributor.authorAzizoğlu, Uğur
dc.contributor.authorAyvaz, Abdurrahman
dc.contributor.authorYılmaz, Semih
dc.contributor.authorKarabörklü, Salih
dc.contributor.authorTemizgül, Rıdvan
dc.date.accessioned2020-05-01T12:10:02Z
dc.date.available2020-05-01T12:10:02Z
dc.date.issued2016
dc.departmentDÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.descriptionKaraborklu, Salih/0000-0003-4737-853X; TEMIZGUL, Ridvan/0000-0002-1033-7067en_US
dc.descriptionWOS: 000381101200010en_US
dc.descriptionPubMed: 27143037en_US
dc.description.abstractIn this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the crylAb into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl beta-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, similar to 130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 mu g g(-1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that crylAb may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND licenseen_US
dc.description.sponsorshipErciyes University Scientific Project UnitErciyes University [FBD11-3634, ONAP-3638]; Bilim, Sanayi ve Teknoloji Bakanligi Turkiye [TGSD-0802]en_US
dc.description.sponsorshipThe authors thank Dr. Mikail Akbulut and Zehra Busra Atciyurt for their valuable assistance. Special thanks to METU Molecular Biology Laboratory for supplying E. coli DH5 alpha. This project was funded by the Erciyes University Scientific Project Unit under the codes of FBD11-3634 and ONAP-3638 and also funded by Bilim, Sanayi ve Teknoloji Bakanligi Turkiye (TGSD-0802).en_US
dc.identifier.doi10.1016/j.bjm.2016.04.011en_US
dc.identifier.endpage602en_US
dc.identifier.issn1517-8382
dc.identifier.issn1678-4405
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage597en_US
dc.identifier.urihttps://doi.org/10.1016/j.bjm.2016.04.011
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5937
dc.identifier.volume47en_US
dc.identifier.wosWOS:000381101200010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Brasileira Microbiologiaen_US
dc.relation.ispartofBrazilian Journal Of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBt SY49-1en_US
dc.subjectGene cloningen_US
dc.subjectRecombinant Cry1Aben_US
dc.subjectInsecticidal activityen_US
dc.titleExpression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insectsen_US
dc.typeArticleen_US

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