The inhibitory effects of Bevacizumab and Dexamethasone on corneal neovascularization
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Drunpp-Sarajevo
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: To investigate the effect of bevacizumab and dexamethasone in the prevention of experimentally induced corneal neovascularization in a rat model. Methods: Thirty male Wistar-Albino rats weighing between 250 g and 300 g were used in the study. Silver nitrate sticks (75% silver nitrate, 25% potassium nitrate) were used to induce chemical cauterization on the corneas of 30 eyes. The rats were randomized to 1 of 5 groups and each group contained 6 rats: Group 1 (control group) without treatment; group 2 received topical 0,1% dexamethasone phosphate twice daily; group 3 received a subconjunctival injection of bevacizumab (0.1 mL, 2.5 mg); group 4 received topical 25mg/ml bevacizumab twice daily, and group 5 was treated with 0.1ml, 2.5 mg of subconjunctival bevacizumab once a day plus topical 25mg/ml bevacizumab twice daily. Corneas were evaluated by operating microscope on the tenth day. Number of major blood vessels was determined. After the examination, enucleation was performed. Histopathologic examinations were made with paraffin block, Hematoxilen-Eosin and Masson's trichrome staining. Blood vessels were counted in the histopathologic cornea sections. Cornea sections were evaluated with regard to the intensity of new vessels, collagen fibers, oedema and fibroblastic activity. Results: The corneal neovascularization in Group 1 were significantly higher than groups 2, 3, 4 and 5 (P<0.05). Corneal neovascularization in bevacizumab treated groups was less than the group treated with topical dexamethasone phosphate and the control group (p<0.05). However, there was no statistically significant difference between the three groups treated with bevacizumab (p>0.05). Masson's trichrome staining in the control group in the region below the epithelium, severe edema and collagen fibers were observed in apparent disorder and separation. The severity of symptoms was less severe in the group treated with topical dexamethasone than the control group. Collagen fibers were organized in bevacizumab treated rats and a marked edema was not observed in them. Conclusion: Bevacizumab and dexamethasone decreased the number of new blood vessels significantly in experimental corneal neovascularization model.
Açıklama
WOS: 000314345000012
Anahtar Kelimeler
Corneal neovascularization, Bevacizumab, Dexamethasone
Kaynak
Healthmed
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
6
Sayı
12
