Effects of Walnut Septum on The Enzyme Pathways Associated with Plasma Cholesterol Level
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Duzce Univ, Fac Medicine
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Objective: Cholesterol is crucial compound that plays pivotal role in cellular function in living organisms. Its excess or deficiency in plasma can lead to destruction and disintegration of cell membrane structure. Maintaining balanced intake of cholesterol in diet and seeking medical treatment, if necessary, can help prevent these negative effects. Furthermore, people often resort to natural and herbal remedies, such as walnut septum. Due to dearth of scientific data regarding effects of walnut septum on cholesterol metabolism, this research was undertaken to explore its potential effects. Methods: Analysis was begun by extracting septum using various solvents. Resulting extracts were then analyzed using GC-MS, and compounds were identified by using an integrated library database. To detect effects of extracts on cholesterol esterase and HMGCoA reductase, a colorimetric method was employed. Results: Monophenol, 2,4-Di-tert-butylphenol, 2,6-Di-tert-butylphenol, ethyl linoleate, and butyl linoleate were some of compounds detected by GC-MS scanning. The highest inhibitions were observed in the enzymatic analysis, with a rate of 3.2% (acetone) in the HMG-CoA reductase analysis and 13.6% (water) in the cholesterol esterase analysis. Conclusions: Although the walnut septum extract contains various chemical compounds, our in vitro analysis data suggest that there is no inhibitory effect at therapeutic level on enzyme pathways that regulate plasma cholesterol levels, namely HMG-CoA reductase and cholesterol esterase. We believe that further research is necessary to comprehensively evaluate its effects on other pathways.
Description
Keywords
Walnut Septum, HMG-CoA Reductase, Cholesterol Esterase, GC-MS, Inhibitory-Activity, Antioxidant, Esterase, Extracts
Journal or Series
Konuralp Tıp Dergisi
WoS Q Value
N/A
Scopus Q Value
Volume
16
Issue
2
