Cloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae)

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dc.contributor.authorYılmaz, Semih
dc.contributor.authorAzizoğlu, Uğur
dc.contributor.authorAyvaz, Abdurrahman
dc.contributor.authorTemizgül, Rıdvan
dc.contributor.authorAtcıyurt, Zehra Büşra
dc.contributor.authorKarabörklü, Salih
dc.date.accessioned2020-04-30T22:40:55Z
dc.date.available2020-04-30T22:40:55Z
dc.date.issued2017
dc.departmentDÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.descriptionKaraborklu, Salih/0000-0003-4737-853X; TEMIZGUL, Ridvan/0000-0002-1033-7067en_US
dc.descriptionWOS: 000398650700013en_US
dc.descriptionPubMed: 28215855en_US
dc.description.abstractBacillus thuringiensis (Berliner) (Bt) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. In this study, a new member of cry2A gene from previously characterized native B. thuringiensis SY49-1 strain was cloned, expressed and used for its activity against Culex pipiens (Diptera: Culicidae) larvae. The sequence analysis of the cloned cry2A gene revealed that it encodes a polypeptide of 633 as residues with 99% identity to Cry2Aa protein with expected molecular weight of 70.7 kDa. Bacillus thuringiensis delta-endotoxin nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Bioassays against last instar larvae of C pipiens indicated that Cry2Aa18 has considerable toxicity with LC50 of 630 jig ml(-1). In order to prevent the spread of infectious diseases mediated by C. pipiens, this newly characterized cry2Aa18 gene could constitute as an important biological control tool for controlling mosquito larvae living in freshwater systems and can be used as a good alternative for minimizing the use of chemicals. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipErciyes University Scientific Project UnitErciyes University [ONAP-3638, FBD11-3634]; Ministry of Industrial Science and Technology, Turkey [TGSD-0802]en_US
dc.description.sponsorshipThe authors thank Dr. Mikail Akbulut for his valuable assistance about molecular issues and Dr. Osman Ibis for next generation sequencing data analysis. This project was funded by Erciyes University Scientific Project Unit under the codes of ONAP-3638 and FBD11-3634 and also funded by Ministry of Industrial Science and Technology, Turkey (TGSD-0802).en_US
dc.identifier.doi10.1016/j.micpath.2017.02.016en_US
dc.identifier.endpage85en_US
dc.identifier.issn0882-4010
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage81en_US
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2017.02.016
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3086
dc.identifier.volume105en_US
dc.identifier.wosWOS:000398650700013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofMicrobial Pathogenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectCloningen_US
dc.subjectBt SY49-1en_US
dc.subjectcry2Aa18en_US
dc.subjectLarvicidal activityen_US
dc.subjectCulex pipiensen_US
dc.titleCloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae)en_US
dc.typeArticleen_US

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