Assessment of genetic diversity and phylogenetic relationships of endangered endemic plant Barbarea integrifolia DC. (Brassicaceae) in Turkey

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dc.contributor.authorFiliz, Ertuğrul
dc.contributor.authorOsma, Etem
dc.contributor.authorKandemir, Ali
dc.contributor.authorTombuloğlu, Hüseyin
dc.contributor.authorTombuloğlu, Güzin
dc.contributor.authorBirbilener, Seda
dc.contributor.authorAydın, Mehtap
dc.date.accessioned2020-04-30T22:39:44Z
dc.date.available2020-04-30T22:39:44Z
dc.date.issued2014
dc.departmentDÜ, Çilimli Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionTombuloglu, Huseyin/0000-0001-8546-2658; Filiz, Ertugrul/0000-0001-9636-6389en_US
dc.descriptionWOS: 000345129500013en_US
dc.description.abstractBarbarea integrifolia DC. ( Brassicaceae) is an endangered and endemic species located in Erzincan and Gumushane provinces of Turkey. In total, 27 individuals from 2 natural populations were assessed using the random amplified polymorphic DNA polymerase chain reaction ( RAPD- PCR) method coupled with sequence analysis of the internal transcribed spacer ( ITS1) rDNA region. Genetic accuracy of RAPD- PCR was tested with 25 RAPD primers, and it resulted in 115 clear and reproducible DNA fragments from 13 RAPD primers. Among these, 76 ( 66.1%) fragments were found to be polymorphic. According to the ITS1 analysis of B. integrifolia populations, the plants exhibited a relatively poor genetic diversity. In total, 60 ITS1 nucleotide sequences from the GenBank database with 2 newly identified ITS1 sequences from the Turkish populations were used to construct a maximum- likelihood tree. The lengths of ITS1 sequences of B. integrifolia were found to be 268 bp for population I and 271 bp for population II. ITS1 sequences of B. integrifolia species showed 97% sequence identity. Phylogenetic analysis revealed that Barbarea species were monophyletic and showed high bootstrap values. B. integrifolia had closer relationships with Cardamineae taxa, including Cardamine, Rorippa, and Armoracia. On the contrary, allied genus Nasturtium species were separated from Cardamineae taxa. The genetic information obtained from this study could be used for the development of conservation strategies not only for B. integrifolia but also for rare and endangered plant species.en_US
dc.identifier.doi10.3906/bot-1403-40en_US
dc.identifier.endpage1181en_US
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1169en_US
dc.identifier.urihttps://doi.org/10.3906/bot-1403-40
dc.identifier.urihttps://hdl.handle.net/20.500.12684/2814
dc.identifier.volume38en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBarbarea integrifoliaen_US
dc.subjectITS1en_US
dc.subjectBrassicaceaeen_US
dc.subjectgenetic diversityen_US
dc.subjectRAPDen_US
dc.subjectendangered speciesen_US
dc.titleAssessment of genetic diversity and phylogenetic relationships of endangered endemic plant Barbarea integrifolia DC. (Brassicaceae) in Turkeyen_US
dc.typeArticleen_US

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