Gentamicin-induced nephrotoxicity in rats ameliorated and healing effects of resveratrol
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Date
2007
Journal Title
Journal ISSN
Volume Title
Publisher
Pharmaceutical Soc Japan
Access Rights
info:eu-repo/semantics/openAccess
Abstract
In this study, we aimed to investigate the possible protective effect of resveratrol on gentamicin induced nephrotoxicity. Experiments were carried out in male Wistar rats weighing 200-250g. Gentamicin sulfate (80 mg/kg per day i.p.), resveratrol (10 mg/kg per day i.p.) and gentamicin together with resveratrol were administered for 6 d. The animals were sacrificed 24 h after the last injection. Urine, blood samples and tissue samples were collected from the animals on the seventh day of the treatment before they were sacrificed. Kidneys were collected for histopathological studies and fixed in 10% buffered formalin solution. Tissue samples were stored at -70 degrees C in liquid nitrogen for the determination of glutathione (GSH), glutathione-S-transferase (GST), malondialdehyde (MDA) and catalase (CAT). Glutathione assay was determined by the method of Beutler et al. GST amounts were measured by the method of Habig et al. Catalase activitiy was tested by Aebi's method and MDA was determined according to Thayer's method. Blood urea level was significantly increased in the gentamicin treated group. The study showed lowered levels of urea and creatinine levels in resveratrol administered groups when compared with gentamicin administered rats, and the difference was statistically significant. It has been determined that resveratrol caused statistically significant decrease in lipid peroxidation and reduced the level of catalase. Histopathological examination showed that resveratrol prevented partly gentamicin induced tubular damage. The results histopathologically demonstrated that resveratrol has a protective effect against gentamicin induced nephrotoxicity, lipid peroxidation and cellular damage in rats.
Description
Silan, Coskun/0000-0002-8352-6571; cengiz, mujgan/0000-0003-1030-5425; silan, coskun/0000-0002-8352-6571
WOS: 000243960400016
PubMed: 17202664
WOS: 000243960400016
PubMed: 17202664
Keywords
resveratrol, gentamicin, nephrotoxicity, lipid peroxidation, antioxidant
Journal or Series
Biological & Pharmaceutical Bulletin
WoS Q Value
Q3
Scopus Q Value
Q2
Volume
30
Issue
1
