In Vitro Effects of Phthalate Mixtures on Colorectal Adenocarcinoma Cell Lines

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dc.contributor.authorDikmen, Begüm Yurdakok
dc.contributor.authorAlpay, Merve
dc.contributor.authorKısmalı, Görkem
dc.contributor.authorFilazi, Ayhan
dc.contributor.authorKuzukıran, Özgür
dc.contributor.authorŞireli, Ufuk Tansel
dc.date.accessioned2020-04-30T23:18:27Z
dc.date.available2020-04-30T23:18:27Z
dc.date.issued2015
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionKUZUKIRAN, OZGUR/0000-0001-9294-2801; Filazi, Ayhan/0000-0002-2800-6215en_US
dc.descriptionWOS: 000368384400003en_US
dc.descriptionPubMed: 26081030en_US
dc.description.abstractAmong endocrine-disrupting chemicals, phthalates are an important concern because of their widespread exposure in humans and environmental contamination. Even though the use of some phthalates has been restricted for toys, some plastics, and food contact materials, exposure to the mixture of these contaminants at very low concentrations in various matrices are still being reported. In the current research, the effects of the mixture of some phthalates were studied. Di-n-butyl phthalate (DBP), n-butyl benzyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisononyl phthalate (DiNP), di-n-octyl phthalate (DNOP), and diisodecyl phthalate (DiDP) were tested on two colorectal adenocarcinoma cell lines; DLD-1 and HT29 were studied as described before. Cells were treated with increasing log concentrations (0.33 ppt to 33.33 ppb) of the phthalate mixture, cell viability/proliferation was measured by MTT and staining with neutral red and crystal violet; lactate dehydrogenase (LDH) activity was measured following 24-h exposure. Cell viability/proliferation increased from phthalate treatment at concentrations less than 33.33 ppt. The phthalate mixture induced increases in HT29 proliferation of 10.94% at 33.33 ppt and 60.87% at 3.33 ppt, whereas this proliferation relation at lower concentrations was not found for DLD1 cells. The present study demonstrates preliminary information regarding the low dose induction of proliferation of the cancer cells by phthalate mixtures. Because non-monotonic dose responses are still being debated, further studies are required to re-evaluate the reference doses defined by governments for phthalates.en_US
dc.identifier.doi10.1615/JEnvironPatholToxicolOncol.2015013256en_US
dc.identifier.endpage123en_US
dc.identifier.issn0731-8898
dc.identifier.issn2162-6537
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage115en_US
dc.identifier.urihttps://doi.org/10.1615/JEnvironPatholToxicolOncol.2015013256
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3327
dc.identifier.volume34en_US
dc.identifier.wosWOS:000368384400003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBegell House Incen_US
dc.relation.ispartofJournal Of Environmental Pathology Toxicology And Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectphthalateen_US
dc.subjectcarcinomaen_US
dc.subjectenvironmental contaminationen_US
dc.subjectreference dosesen_US
dc.subjectcell proliferationen_US
dc.subjectmixture effecten_US
dc.titleIn Vitro Effects of Phthalate Mixtures on Colorectal Adenocarcinoma Cell Linesen_US
dc.typeArticleen_US

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