Probiotics ameliorates LPS induced neuroinflammation injury on A beta 1-42, APP, gamma-beta secretase and BDNF levels in maternal gut microbiota and fetal neurodevelopment processes

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dc.authoridKAR, Ezgi/0000-0003-2134-4067
dc.authoridKAR, FATIH/0000-0001-8356-9806
dc.authorwosidKAR, Ezgi/AAR-1992-2020
dc.contributor.authorKar, Fatih
dc.contributor.authorHacıoğlu, Ceyhan
dc.contributor.authorKar, Ezgi
dc.contributor.authorDonmez, Dilek Burukoglu
dc.contributor.authorKanbak, Güngör
dc.date.accessioned2023-07-26T11:53:54Z
dc.date.available2023-07-26T11:53:54Z
dc.date.issued2022
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.description.abstractThe gut microbiota influences brain development and functioning through the gut-brain axis. This is first study regulate maternal gut microbiota and fetal neurodevelopment processes by using probiotics such as Bifidobacterium bifidum (BIF) and Lactobacillus salivarius (LAC) in the prenatal period. In this study, Wistar Albino female rats were divided into five groups; Control, lipopolysaccharide (LPS, 100 mu g/kg), LPS + LAC, LPS + BIF and LPS + LAC + BIF (4 x 10(9) ml CFU). Maternal rats were given probiotics for 21 days. Inflammation was induced by lipopolysaccharide (LPS), on the 17th day of pregnancy. After birth, the brain tissues of the maternal and neonatal rats were removed and their blood was collected. Fecal calprotectin levels of pregnant rats were measured as an important biomarker in determining intestinal flora disruption. Calprotectin levels were high in LPS group (p < 0.05). A beta 1-42, APP, gamma secretase and beta- secretase levels were higher in both maternal and neonatal LPS groups (p < 0.05). These levels were statistically decreased in the probiotic groups compared to the LPS group, as demonstrated in both biochemical and histological analyzes (p < 0.05). While BDNF mRNA expression decreased in LPS groups, APP level increased in the same group. The difference between groups in mRNA expressions in the neonatal brain tissues was similar to maternal brain tissues. What's more, BDNF/actin and APP/actin rates were proven by western blot and the damage caused by neuroinflammation in the brain tissue and the preservation of the intestinal microbiota were visualized histopathologically on the morphological structures in all groups. It will shed light on new therapeutic strategies for the impact of the use of probiotics on the neurodevelopmental processes of the neonatal against LPS-induced inflammatory responses and impaired gut microbiota in the prenatal period.en_US
dc.description.sponsorshipEskisehir Osmangazi University Scientific Research Projects Commission [2019-1915]en_US
dc.description.sponsorshipThis study was supported by Eskisehir Osmangazi University Scientific Research Projects Commission. (Project No: 2019-1915).en_US
dc.identifier.doi10.1007/s11011-022-00964-z
dc.identifier.endpage1399en_US
dc.identifier.issn0885-7490
dc.identifier.issn1573-7365
dc.identifier.issue5en_US
dc.identifier.pmid35312928en_US
dc.identifier.scopus2-s2.0-85126776948en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1387en_US
dc.identifier.urihttps://doi.org/10.1007/s11011-022-00964-z
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12653
dc.identifier.volume37en_US
dc.identifier.wosWOS:000771363600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorHacıoğlu, Ceyhan
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofMetabolic Brain Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectMicrobiota; Bifidobacterium; Lactobacillus; Lipopolysaccharide (Lps); Inflammation; Alzheimer's Diseaseen_US
dc.subjectEndotoxin; Lactobacillus; Infection; Disease; Bifidobacterium; Dysfunction; Impairment; Cytokine; Dementia; Anxietyen_US
dc.titleProbiotics ameliorates LPS induced neuroinflammation injury on A beta 1-42, APP, gamma-beta secretase and BDNF levels in maternal gut microbiota and fetal neurodevelopment processesen_US
dc.typeArticleen_US

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