Probiotics ameliorates LPS induced neuroinflammation injury on A beta 1-42, APP, gamma-beta secretase and BDNF levels in maternal gut microbiota and fetal neurodevelopment processes
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer/Plenum Publishers
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The gut microbiota influences brain development and functioning through the gut-brain axis. This is first study regulate maternal gut microbiota and fetal neurodevelopment processes by using probiotics such as Bifidobacterium bifidum (BIF) and Lactobacillus salivarius (LAC) in the prenatal period. In this study, Wistar Albino female rats were divided into five groups; Control, lipopolysaccharide (LPS, 100 mu g/kg), LPS + LAC, LPS + BIF and LPS + LAC + BIF (4 x 10(9) ml CFU). Maternal rats were given probiotics for 21 days. Inflammation was induced by lipopolysaccharide (LPS), on the 17th day of pregnancy. After birth, the brain tissues of the maternal and neonatal rats were removed and their blood was collected. Fecal calprotectin levels of pregnant rats were measured as an important biomarker in determining intestinal flora disruption. Calprotectin levels were high in LPS group (p < 0.05). A beta 1-42, APP, gamma secretase and beta- secretase levels were higher in both maternal and neonatal LPS groups (p < 0.05). These levels were statistically decreased in the probiotic groups compared to the LPS group, as demonstrated in both biochemical and histological analyzes (p < 0.05). While BDNF mRNA expression decreased in LPS groups, APP level increased in the same group. The difference between groups in mRNA expressions in the neonatal brain tissues was similar to maternal brain tissues. What's more, BDNF/actin and APP/actin rates were proven by western blot and the damage caused by neuroinflammation in the brain tissue and the preservation of the intestinal microbiota were visualized histopathologically on the morphological structures in all groups. It will shed light on new therapeutic strategies for the impact of the use of probiotics on the neurodevelopmental processes of the neonatal against LPS-induced inflammatory responses and impaired gut microbiota in the prenatal period.
Açıklama
Anahtar Kelimeler
Microbiota; Bifidobacterium; Lactobacillus; Lipopolysaccharide (Lps); Inflammation; Alzheimer's Disease, Endotoxin; Lactobacillus; Infection; Disease; Bifidobacterium; Dysfunction; Impairment; Cytokine; Dementia; Anxiety
Kaynak
Metabolic Brain Disease
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
37
Sayı
5
