DNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clones

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dc.contributor.authorÖzyiğit, İbrahim İlker
dc.contributor.authorKaval, Ali
dc.contributor.authorÇakır, Ernaz Altundağ
dc.contributor.authorVardar, Filiz
dc.date.accessioned2020-05-01T09:11:27Z
dc.date.available2020-05-01T09:11:27Z
dc.date.issued2019
dc.departmentDÜ, Fen-Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionVardar, Filiz/0000-0002-1051-5628; Ozyigit, Ibrahim Ilker/0000-0002-0825-5951en_US
dc.descriptionWOS: 000470332600011en_US
dc.descriptionPubMed: 30911971en_US
dc.description.abstractAluminum (Al) is one of the most important stress factors that reduce plant productivity in acidic soils. Present work thereby analyzed Al-induced genomic alterations in Bryophyllum daigremontianum clones using RAPD and ISSR markers, and investigated responding changes in photosynthetic pigment (chlorophyll a, b, a/b, total chlorophyll and carotenoid) contents and total soluble protein amounts in plant leaves. The main reason for the use of bulbiferous spurs originated clone plants was to increase reliability and acceptability of RAPD and ISSR techniques in DNA fingerprinting. Raised 40 clone plants were divided into five separate groups each with eight individuals and each experimental group was watered with 0 (control), 0 (acid control), 50, 100 and 200 mu M AlCl3-containing Hoagland solutions on alternate days for two and a half months. All plant soils except control group were sprayed with 0.2% sulfuric acid following watering days and this contributed acidic characteristic (pH 4.8) to soil structure. Increase in Al concentrations were accompanied by an increase in total soluble protein amounts, a decrease in photosynthetic pigment contents, and with appearance, disappearance and intensity changes at RAPD and ISSR band profiles. Out of tested RAPD1-25 and ISSR1-15 primers, RAPD8, RAPD9, ISSR2 and ISSR7 primers produced reproducible band profiles that were distinguishable between treatment and control groups. Findings showed that RAPD and ISSR fingerprints have been useful biomarkers for investigation of plant genotoxicity, especially in clone plants. Moreover, if these fingerprints are integrated with other physiological parameters they could become more powerful tools in ecotoxicology.en_US
dc.description.sponsorshipDuzce University Research FundDuzce University [2015.05.01.376]en_US
dc.description.sponsorshipThis project is supported by Duzce University Research Fund. Project Number: 2015.05.01.376.en_US
dc.identifier.doi10.1007/s11033-019-04714-wen_US
dc.identifier.endpage2711en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2703en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-019-04714-w
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5602
dc.identifier.volume46en_US
dc.identifier.wosWOS:000470332600011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRAPDen_US
dc.subjectISSRen_US
dc.subjectClone planten_US
dc.subjectGenotoxicityen_US
dc.subjectAcidic soilen_US
dc.subjectDNA fingerprintingen_US
dc.titleDNA fingerprinting and assessment of some physiological changes in Al-induced Bryophyllum daigremontianum clonesen_US
dc.typeArticleen_US

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