Curcumin Acts as Post-protective Effects on Rat Hippocampal Synaptosomes in a Neuronal Model of Aluminum-Induced Toxicity

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dc.contributor.authorKar, Fatih
dc.contributor.authorHacıoğlu, Ceyhan
dc.contributor.authorUslu, Sema
dc.contributor.authorKanbak, Güngör
dc.date.accessioned2020-05-01T09:11:15Z
dc.date.available2020-05-01T09:11:15Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000477589200021en_US
dc.descriptionPubMed: 31264110en_US
dc.description.abstractThe neurotoxic effects of aluminum are generally associated with reduced antioxidant capacity, increased oxidative stress and apoptosis, which lead to the induction of neurodegenerative processes. Curcumin has a lipophilic polyphenol character and effects of antioxidant and anti-apoptotic. The present study was undertaken to examine possible aluminum exposure in rats brain synaptosomes and to investigate whether protective and therapeutic effects of curcumin on biochemical and morphological changes in both pre- and post-treated groups. Aluminum chloride (AlCl3) at 50 mu M concentration and curcumin at 5 and 10 mu g/mL doses were applied to hippocampal synaptosomes of rats according to experimental design. Biochemical effects were evaluated by MTT cytotoxicity, malondialdehyde (MDA) levels, nitric oxide (NO) levels, glutathione (GSH) levels, caspase 3 activities, cytochrome c levels, DNA fragmentation values and protein levels. Morphological examinations were done by TEM analysis. AlCI3 exposure in the synaptosomes enhanced oxidative stress, triggered apoptosis and caused ultrastructural alterations which were well reflected in the TEM images. Curcumin pre-treatment slightly ameliorated the MDA levels, NO levels, cytochrome c levels and caspase 3 activities in AlCI3-exposed synaptosomes, but these results were not statistically significant. Furthermore, curcumin post-treatment significantly improved oxidative damage and morphological alterations, and suppressed cytochrome c and caspase 3 activities. Taken together, our data showed that curcumin had more therapeutic effects than protective effects in AlCI3-induced neurotoxicity. Nevertheless, the therapeutic (post-protective) effects of curcumin should be further investigated in in vivo neurodegenerative models involving behavioral tests.en_US
dc.identifier.doi10.1007/s11064-019-02839-9en_US
dc.identifier.endpage2029en_US
dc.identifier.issn0364-3190
dc.identifier.issn1573-6903
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2020en_US
dc.identifier.urihttps://doi.org/10.1007/s11064-019-02839-9
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5428
dc.identifier.volume44en_US
dc.identifier.wosWOS:000477589200021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofNeurochemical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAluminum chlorideen_US
dc.subjectCurcuminen_US
dc.subjectOxidative stressen_US
dc.subjectApoptosisen_US
dc.subjectMorphological alterationen_US
dc.subjectHippocampal synaptosomesen_US
dc.titleCurcumin Acts as Post-protective Effects on Rat Hippocampal Synaptosomes in a Neuronal Model of Aluminum-Induced Toxicityen_US
dc.typeArticleen_US

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