Investigation of Biofilm Formation, Anti-Quorum Sensing Activity and Antimicrobial Resistance in Corynebacterium Species Isolated from Clinical Samples

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dc.authorscopusid57223034692en_US
dc.authorscopusid59107961500en_US
dc.authorscopusid58291089900en_US
dc.authorscopusid58751426000en_US
dc.authorscopusid25229718700en_US
dc.authorscopusid57811073900en_US
dc.authorscopusid55992119800en_US
dc.contributor.authorKeskin, B.H.
dc.contributor.authorŞahin, I.
dc.contributor.authorKahraman, G.
dc.contributor.authorDuran, P.K.
dc.contributor.authorDülger, G.
dc.contributor.authorDurmuş, M.A.
dc.contributor.authorCeylan, A.N.
dc.date.accessioned2024-08-23T16:07:22Z
dc.date.available2024-08-23T16:07:22Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractAn increasing number of different clinical infections caused by Corynebacteria have been reported in the last decade. The aim of this study was to evaluate the antibiotic resistance rates, biofilm formation capacities and to investigate the ‘‘anti-quorum-sensing (anti-QS)’’ activities of corynebacteria, which were divided into three groups according to the type of growth in culture (pure, with another pathogenic bacterium and polymicrobial growth). In total 240 Corynebacterium spp. isolates from different clinical specimens sent to the medical microbiology laboratories of Düzce University Faculty of Medicine Hospital and Başakşehir Çam and Sakura City Hospital between June 2021 and June 2022 were classified into three groups: pure, isolated with another pathogen and polymicrobial, according to their growth patterns in culture. Bacteria were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Biotyper (Bruker, Germany) at an external centre. Antibiotic susceptibilities were determined by disc diffusion method and for vancomycin broth microdilution method was used. Results were interpreted according to EUCAST recommendations. The biofilm-forming properties of the isolates were determined quantitatively. Bioactive components of 17 isolates with strong biofilm formation were extracted and anti-QS activity was determined by agar diffusion method using Chromobacterium violaceum ATCC 12472 strain and then violacein pigment production was measured quantitatively. Of the 240 Corynebacterium spp. isolates, 138 (58%) were pure, 52 (22%) were isolated with another pathogen and 50 (20%) were part of a polymicrobial infection. Of the isolates, 140 were identified as Corynebacterium striatum, 34 as Corynebacterium amycolatum and 24 as Corynebacterium afermentans. When the antibiotic resistance rates of the Corynebacterium isolates were analysed according to the groups, the resistance rates to rifampicin and tetracycline antibiotics were found to be statistically significantly lower in the polymicrobial group than in the other groups. The resistance rates to penicillin, clindamycin, ciprofloxacin, moxifloxacin, rifampicin, tetracycline and linezolid were 96.7%, 88.3%, 86.3%, 73.8%, 62.5%, 59.2% and 0.8%, respectively. While all isolates were susceptible to vancomycin, linezolid resistance was detected in two C.afermentans isolates. When the biofilm formation ability was analysed, it was observed that 87 (36.3%) isolates formed biofilm. The biofilm formation rate of the isolates in the polymicrobial growth group was lower than the other two groups. The anti-QS activity of 17 isolates with strong biofilm formation was investigated and none of the Corynebacterium extracts tested were found to have anti-QS activity (inhibition of violacein pigment production without inhibiting bacterial growth) in the QS study with C.violaceum, whereas five isolate extracts had antibacterial activity (inhibition of bacterial growth). Four of the bacterial extracts with antimicrobial activity belonged to C.amycolatum and one to C.afermentans. In conclusion, when both antibiotic resistance rates and biofilm formation rates were analysed, the corynebacteria growing in culture with another pathogen showed similar characteristics to the corynebacteria growing as a pure culture. Therefore, it was thought that corynebacteria growing with another pathogen should not be ignored. In addition, the antimicrobial effects of some corynebacterial extracts suggested that more QS studies should be carried out with microbiota bacteria. © 2024 Ankara Microbiology Society. All rights reserved.en_US
dc.identifier.doi10.5578/mb.20249704
dc.identifier.endpage258en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid39046207en_US
dc.identifier.scopus2-s2.0-85199346716en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage239en_US
dc.identifier.trdizinid1250538en_US
dc.identifier.urihttps://doi.org/10.5578/mb.20249704
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1250538
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14622
dc.identifier.volume58en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Societyen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectbiofilmen_US
dc.subjectCorynebacteriumen_US
dc.subjectquorum sensingen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectBiofilmsen_US
dc.subjectCorynebacteriumen_US
dc.subjectCorynebacterium Infectionsen_US
dc.subjectDrug Resistance, Bacterialen_US
dc.subjectHumansen_US
dc.subjectIndolesen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectQuorum Sensingen_US
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.subjectantiinfective agenten_US
dc.subjectindole derivativeen_US
dc.subjectantibiotic resistanceen_US
dc.subjectbiofilmen_US
dc.subjectCorynebacteriumen_US
dc.subjectCorynebacterium infectionen_US
dc.subjectdrug effecten_US
dc.subjectgrowth, development and agingen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectmatrix-assisted laser desorption-ionization mass spectrometryen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectquorum sensingen_US
dc.titleInvestigation of Biofilm Formation, Anti-Quorum Sensing Activity and Antimicrobial Resistance in Corynebacterium Species Isolated from Clinical Samplesen_US
dc.title.alternativeKlinik Örneklerden İzole Edilen Corynebacterium Türlerinde Biyofilm Oluşumunun, Anti-Quorum Sensing Aktivitesinin ve Antimikrobiyal Direnç Durumunun Araştırılmasıen_US
dc.typeArticleen_US

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