Biodegradable microspherical implants containing teicoplanin for the treatment of methicillin-resistant Staphylococcus aureus osteomyelitis

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dc.contributor.authorOrhan, Zafer
dc.contributor.authorCevher, Erdal
dc.contributor.authorYıldız, Ayça
dc.contributor.authorAhıskalı, Rengin
dc.contributor.authorŞensoy, Demet
dc.contributor.authorMülazımoğlu, Lütfiye
dc.date.accessioned2020-04-30T22:40:11Z
dc.date.available2020-04-30T22:40:11Z
dc.date.issued2010
dc.departmentDÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000271920400020en_US
dc.descriptionPubMed: 19434413en_US
dc.description.abstractThe aim of this study was to prepare poly(d,l-lactide-co-glycolide) (PLGA) microspherical implants containing teicoplanin (TCP) using a double emulsion solvent evaporation method and to evaluate its efficacy for the local treatment of chronic osteomyelitis. The particle size and distribution, morphological characteristics, thermal behaviour, drug content, encapsulation efficiency and in vitro release assessments of the formulations were carried out. Sterile TCP-PLGA microspheres were implanted in the proximal tibia of rats with methicillin resistant Staphylococcus aureus (MRSA) osteomyelitis. After 3 weeks of treatment, bone samples were analysed with a microbiological assay and evaluated histopathologically. Microspheres between the size ranges of 2.01 and 3.91 mu m were obtained. Production yield of all formulations was found to be higher than 82% and encapsulation efficiencies of 33.6-69.8% were obtained. DSC thermogram showed that the TCP was in an amorphous state in microspheres. In vitro drug release studies had indicated that the drug release rate of microspheres was decreased upon increasing the polymer:drug ratio. Based on the in vivo data, rats treated with implants and intramuscular injection showed 1.7 x 10(3) +/- A 1.3 x 10(3) and 5.8 x 10(4) +/- A 5.3 x 10(4) colony forming unit of MRSA in 1 g bone samples (CFU/g), respectively (P < 0.01). The in vitro and in vivo studies had shown that the TCP-PLGA microspheres were effective for the treatment of chronic osteomyelitis in an animal experimental model. Hence, these microspheres may be potentially useful in the clinical setting with the need for further investigation for optimal dosing of TCP-PLGA microspheres.en_US
dc.identifier.doi10.1007/s00402-009-0886-9en_US
dc.identifier.endpage142en_US
dc.identifier.issn0936-8051
dc.identifier.issue1en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage135en_US
dc.identifier.urihttps://doi.org/10.1007/s00402-009-0886-9
dc.identifier.urihttps://hdl.handle.net/20.500.12684/2925
dc.identifier.volume130en_US
dc.identifier.wosWOS:000271920400020en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives Of Orthopaedic And Trauma Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTCP-PLGA implantsen_US
dc.subjectOsteomyelitisen_US
dc.subjectMRSAen_US
dc.titleBiodegradable microspherical implants containing teicoplanin for the treatment of methicillin-resistant Staphylococcus aureus osteomyelitisen_US
dc.typeArticleen_US

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