Prevalence of Helicobacter pylori in symptomatic patients and detection of clarithromycin resistance using melting curve analysis

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dc.contributor.authorKaya, Ayşe Demet
dc.contributor.authorÖztürk, C. Elif
dc.contributor.authorAkcan, Yusuf
dc.contributor.authorBehçet, Mustafa
dc.contributor.authorKarakoç, A. Esra
dc.contributor.authorYücel, Mihriban
dc.contributor.authorTuncer, Serdar
dc.date.accessioned2020-04-30T23:21:35Z
dc.date.available2020-04-30T23:21:35Z
dc.date.issued2007
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000248245200003en_US
dc.descriptionPubMed: 24683206en_US
dc.description.abstractBackground: Clarithromycin is often a component of combination therapies for Helicobacter pylori eradication; however, increases in resistance rates have decreased the success of the treatment. Objective: This study was designed to determine the prevalence of H pylori infection in symptomatic patients and to detect clarithromycin resistance rates using melting curve analysis. Methods: Patients scheduled for upper endoscopy at the Endoscopy Unit of the Department of Gastroenterology, Duzce University, Medical Faculty Hospital, Konuralp/Duzce, Turkey, were assessed for enrollment in the study. Two pairs of gastric biopsy specimens (antrum and corpus) were obtained from each study patient. Histopathologic examination, rapid urease test, culture, and polymerase chain reaction (PCR) of the specimens were used to identify H pylori infection. Clarithromycin resistance was detected using melting curve analysis. Results: Seventy-five patients (41 women, 34 men; mean [SD] age, 42.6 [14.5] years [range, 17-70 years]) were included in the study. Using histopathology and rapid urease test, H pylori was detected in 40 (53.3%) of the 75 specimens. H pylori was detected using PCR in 40 (53.3%) specimens and by culture in 10 (13.3%) specimens. The specificity and sensitivity of PCR and culture were interpreted by comparing them with the results of histopathologic examination and urease tests. The specificity and sensitivity of PCR were 68.6% and 72.5%, respectively, and the specificity and sensitivity of culture were 97.1% and 22.5%, respectively. Of the 40 isolates, 21 (52.5%) were susceptible to clarithromycin, 12 (30.0%) were resistant, and a mixed susceptibility pattern was detected in 7 (17.5%) specimens. H pylori isolates from 19 (79.2%) of the 24 patients who had formerly used clarithromycin showed clarithromycin resistance. Conclusions: The prevalence of H pylori infection was 53.3% for the symptomatic patients in this study, and 47.5% of the isolates showed clarithromycin resistance using melting curve analysis. The PCR-based system used in this study was accurate for the detection of H pylori infection as well as clarithromycin susceptibility testing directly in biopsy specimens.en_US
dc.identifier.doi10.1016/j.curtheres.2007.06.001en_US
dc.identifier.endpage160en_US
dc.identifier.issn0011-393X
dc.identifier.issn1879-0313
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage151en_US
dc.identifier.urihttps://doi.org/10.1016/j.curtheres.2007.06.001
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4217
dc.identifier.volume68en_US
dc.identifier.wosWOS:000248245200003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofCurrent Therapeutic Research-Clinical And Experimentalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHelicobacter pylorien_US
dc.subjectclarithromycin resistanceen_US
dc.subjectmelting curve analysisen_US
dc.subjectPCRen_US
dc.titlePrevalence of Helicobacter pylori in symptomatic patients and detection of clarithromycin resistance using melting curve analysisen_US
dc.typeArticleen_US

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