Is one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining method

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dc.contributor.authorÇolakoğlu, Serdar
dc.contributor.authorSarıtaş, Ayhan
dc.contributor.authorEröz, Recep
dc.contributor.authorOktay, Murat
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorAköz, A.
dc.contributor.authorKandiş, Hayati
dc.date.accessioned2020-04-30T23:18:45Z
dc.date.available2020-04-30T23:18:45Z
dc.date.issued2015
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionYaykasli, Kursat/0000-0001-7550-6370; Saritas, Ayhan/0000-0002-4302-1093; Saritas, Ayhan/0000-0002-4302-1093; Kaya, Ertugrul/0000-0003-0081-682X; Kandis, Hayati/0000-0001-9151-6050en_US
dc.descriptionWOS: 000346643700003en_US
dc.descriptionPubMed: 24812150en_US
dc.description.abstractIn carbon monoxide (CO) poisoning, CO affects the oxygen-carrying capacity of the hemoglobin molecule. Nucleolar-organizing regions (NORs) are genetic loci on chromosomes that are composed of ribosomal DNA and proteins. NORs can be stained with silver. A total of 18 rats were exposed to CO in three different concentrations (1000, 3000, and 5000 ppm) with 6 rats as controls. The animals were euthanized 7 days after CO intoxication. Lung tissues were taken, embedded in paraffin blocks, and sectioned at 5 m thickness. Argyrophilic nucleolar-organizing region (AgNOR) staining was carried out. One hundred nuclei per individual were evaluated, and total AgNOR number per total nuclear number and total AgNOR area per nuclear area (TAA/NA) for each nucleus were analyzed. The CO exposure groups had significantly higher TAA/NA values and AgNOR numbers than the control group (p < 0.05). Although the differences between 1000 ppm and the other two CO-exposed groups were meaningful (p < 0.05) in the TAA/NA values, there were no differences among the CO exposure groups for the AgNOR number (p > 0.05). The increase in TAA/NA value depends on the increase in the CO exposure. Significant correlations between both the AgNOR values and histopathological scoring methods were found. Therefore, AgNOR staining method may be used as an indirect indicator for evaluating the degree of cell damage rate.en_US
dc.identifier.doi10.1177/0960327114531994en_US
dc.identifier.endpage31en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage24en_US
dc.identifier.urihttps://doi.org/10.1177/0960327114531994
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3509
dc.identifier.volume34en_US
dc.identifier.wosWOS:000346643700003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRaten_US
dc.subjectlungen_US
dc.subjectcarbon monoxide intoxicationen_US
dc.subjectAgNORen_US
dc.titleIs one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining methoden_US
dc.typeArticleen_US

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