Betaine suppresses cell proliferation by increasing oxidative stress-mediated apoptosis and inflammation in DU-145 human prostate cancer cell line

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dc.contributor.authorKar, Fatih
dc.contributor.authorHacıoğlu, Ceyhan
dc.contributor.authorKaçar, Sedat
dc.contributor.authorŞahintürk, Varol
dc.contributor.authorKanbak, Güngör
dc.date.accessioned2020-04-30T22:40:08Z
dc.date.available2020-04-30T22:40:08Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionKacar, Sedat/0000-0002-0671-8529; kar, fatih/0000-0001-8356-9806; Sahinturk, Varol/0000-0003-2317-3644en_US
dc.descriptionWOS: 000483691000002en_US
dc.descriptionPubMed: 31368044en_US
dc.description.abstractProstate cancer is the main cause of cancer-related mortality in men around the world and an important health problem. DU-145 human prostate cancer cells provide an opportunity to investigate prostate cancer. Betaine has a number of anticancer effects, such as inactivation of carcinogens, inhibition of cancer cell proliferation, angiogenesis, and metastasis. However, there is no study investigating the effects of betaine on DU-145 cells. The aim of this study was to evaluate the effects of different concentrations of betaine on the oxidative stress, apoptosis, and inflammation on DU-145 cells. Firstly, we proved the cytotoxic activity of betaine (0 to 150 mg/ml) on DU-145 cells by using 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and defined the optimal concentration of betaine. Then, by employing the doses found in MTT, the levels of antioxidant (GSH, SOD, CAT, and TAS) and oxidant (MDA and TOS) molecules, pro-inflammatory cytokines (TNF-a and IL-6), apoptotic proteins (CYCS and CASP3), and DNA fragmentation were measured. Morphological changes and apoptosis were evaluated using H&E technique, Bax and Bcl-2 immunohistochemistry. Results suggested that betaine caused oxidative stress, inflammation, inhibition of cell growth, apoptosis, and morphological alterations in DU-145 cells dose-dependently. Furthermore, treatments with increasing betaine concentrations decreased the antioxidant levels in cells. We actually revealed that betaine, known as an antioxidant, may prevent cell proliferation by acting as an oxidant in certain doses. In conclusion, betaine may act as a biological response modifier in prostate cancer treatment in a dose-dependent manner.en_US
dc.identifier.doi10.1007/s12192-019-01022-xen_US
dc.identifier.endpage881en_US
dc.identifier.issn1355-8145
dc.identifier.issn1466-1268
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage871en_US
dc.identifier.urihttps://doi.org/10.1007/s12192-019-01022-x
dc.identifier.urihttps://hdl.handle.net/20.500.12684/2911
dc.identifier.volume24en_US
dc.identifier.wosWOS:000483691000002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCell Stress & Chaperonesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBetaineen_US
dc.subjectOxidative stressen_US
dc.subjectApoptosisen_US
dc.subjectCell morphologyen_US
dc.subjectDU-145 prostate cancer cellen_US
dc.titleBetaine suppresses cell proliferation by increasing oxidative stress-mediated apoptosis and inflammation in DU-145 human prostate cancer cell lineen_US
dc.typeArticleen_US

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