The effects of radioprotectant and potential antioxidant agent amifostine on the structure and dynamics of DPPC and DPPG liposomes

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dc.contributor.authorArslan, Gülgün Çakmak
dc.contributor.authorSevercan, Feride
dc.date.accessioned2020-04-30T23:33:30Z
dc.date.available2020-04-30T23:33:30Z
dc.date.issued2019
dc.departmentDÜ, Fen-Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionCakmak-Arslan, Gulgun/0000-0003-4326-1780en_US
dc.descriptionWOS: 000470339300021en_US
dc.descriptionPubMed: 31028720en_US
dc.description.abstractAgents capable of scavenging ROS have attracted attention recently because of their potential use as anti oxidative agents. Amifostine, a ROS scavenger, has the potential to be used as an antioxidant in therapeutic applications. In this study, the effect of amifostine on neutral zwitterionic dipalmitoylphosphatidylcholine (DPPC) and anionic dipalmitoylphosphatidylglycerol (DPPG) model membranes' structure and dynamics is aimed to be examined by Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Our results revealed that amifostine at concentrations used (1-24 mol%) does not induce any important alteration in the shape of phase transition curve and phase transition temperature in the DPPC and DPPG membranes. High concentrations of amifostine slightly increased the acyl chain flexibility of DPPC membranes in the liquid crystalline phase and DPPG membranes in the gel phase. A lessening in the dynamics of DPPC liposomes was observed for all concentrations of amifostine in both phases but slight dual effect was observed only in the gel phase as a decrease in dynamics at low concentrations and an increase at higher concentrations of amifostine in DPPG liposomes. Additionally, strong hydrogen bonding was observed for both C=O and PO2- groups in case of DPPC and for PO2- groups in case of DPPG. Dehydration around the C=O regions occurred in case of DPPG. Accordingly, amifostine is proposed to be interacting strongly with zwitterionic and negatively charged membrane head groups and glycerol backbone in all concentrations and because of this interaction it causes some changes in lipid order and dynamics especially at high concentrations.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-2939]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technical Research Council of Turkey (TUBITAK) [grant number: SBAG-2939].en_US
dc.identifier.doi10.1016/j.bbamem.2019.04.009en_US
dc.identifier.endpage1251en_US
dc.identifier.issn0005-2736
dc.identifier.issn1879-2642
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1240en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbamem.2019.04.009
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4983
dc.identifier.volume1861en_US
dc.identifier.wosWOS:000470339300021en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofBiochimica Et Biophysica Acta-Biomembranesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmifostineen_US
dc.subjectDPPCen_US
dc.subjectDPPGen_US
dc.subjectDrug-membrane interactionen_US
dc.subjectFTIR spectroscopyen_US
dc.subjectDSCen_US
dc.titleThe effects of radioprotectant and potential antioxidant agent amifostine on the structure and dynamics of DPPC and DPPG liposomesen_US
dc.typeArticleen_US

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