Comparison of peripheral nerve repair using ethylcyanoacrylate and conventional suture technique in a rat sciatic nerve injury model
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Tarih
2020
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Dergi ISSN
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Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The aim of this study was to compare the outcomes of primary nerve repair using either ethyl-cyanoacrylate orconventional microsuture technique in a rat peripheral nerve injury model.Methods: In this study, a total of 30 Wistar Albino rats weighing between 220 and 275 g were used. The rats were randomlydivided into three groups (10 in each), including one control (group 1) and two experimental groups (group 2, conventionalmicrosuture repair; group 3, cyanoacrylate repair). In each group, the sciatic nerve was identified and transected. No furtherintervention was performed in group 1. The nerve was repaired using the epineural technique with a 10/0 atraumaticnylon in group 2 and synthetic cyanoacrylate adhesive in group 3. At the fifth postoperative week, needle electromyography(EMG) was performed to measure distal latency, combined muscle action potential (CMAP), and motor nerve conductionvelocity (MNCV). Following the EMG recordings, animals were euthanized. Nerve samples were collected to evaluate vacuolardegeneration, fibrosis, and foreign body reaction histopathologically.Results: In the EMG analysis, mean distal latency was significantly shorter in group 1 (0.85±0.09 ms) than in groups 2(1.17±0.25 ms) (p=0.0052) and 3 (1.14±0.14 ms) (p=0.0026) while no significant differences existed between groups 2 and3 (p>0.9999). The mean CMAP was greater in group 1 (10.5±0.35 mV) than in groups 2 (2.86±1.28 mV) (p=0.011) and 3(2.16±1.34 mV) (p=0.0002), but there was no significant difference between groups 2 and 3 (p>0.9999). The mean MNCVwas 53.5±5.95, 39.62±7.31, and 39.84±4.73 mm/sec in groups 1, 2, and 3, respectively. There was a significant differencebetween groups 1 and 2 (p=0.0052) and between 1 and 3 (p=0.0026), but not between 2 and 3 (p>0.9999). In the histopathologicalevaluation, the mean vacuolar degeneration score was 0, 2.12, and 1.88 in groups 1, 2, and 3, respectively. No obviousdifference was observed between groups 2 and 3 (p=0.743). The mean fibrosis score was 0, 1.62, and 1.77 in groups 1, 2,and 3, respectively. There was no significant difference between groups 2 and 3 (p=0.888). The mean foreign body reactionscore was 0, 2.5, and 2.44 in groups 1, 2, and 3, respectively. No difference was present between groups 2 and 3 (p=0.743).Conclusion: Primary nerve repair using the cyanoacrylate adhesive may provide similar electrophysiological and histopathologicalresults as compared to the conventional microsuture repair.
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Anahtar Kelimeler
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Kaynak
Acta Orthopaedica et Traumatologica Turcica
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
54
Sayı
3
