Pyrrolidine dithiocarbamate attenuates the development of monocrotaline-induced pulmonary arterial hypertension
Yükleniyor...
Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Gmbh, Urban & Fischer Verlag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We aimed to demonstrate the potential protective effects of pyrrolidine dithiocarbamate (PDTC) on monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH). Adult male rats were randomly assigned to 4 groups: control group, MCT-treated rats only, MCT-injected rats treated with PDTC, and PDTC-treated rats only. Blood and tissue samples were collected after the sacrifice. Levels of malondialdehyde (MDA) were measured by using the thiobarbituric acid method. Total antioxidant status (TAS) was determined using a commercially available ImAnOx kit. A histopathological evaluation was accomplished by scoring the degree of severity. Endothelial damage of the main pulmonary artery was evaluated by immunohistochemical labeling of endothelial cells using anti-rat endothelial cell antigen 1 (RECA-1) antibody. MCT-induced right ventricular hypertrophy (RVH) was reduced significantly in the MCT + PDTC-treated group. MDA levels were significantly lowered in the MCT + PDTC-treated group. TAS was significantly higher in the MCT + PDTC-treated group when compared with the rats with PAH. Histopathological examination demonstrated that PDTC treatment reduced the development of inflammation, hemorrhage and congestion, and collagen deposition. In conclusion, PDTC attenuated PAH and protected pulmonary endothelium in rats administered MCT. These findings suggest that PDTC treatment may provide a new effective therapeutic approach in the treatment of PAH. (C) 2013 Elsevier GmbH. All rights reserved.
Açıklama
Silan, Coskun/0000-0002-8352-6571; silan, coskun/0000-0002-8352-6571
WOS: 000321090000006
PubMed: 23582365
WOS: 000321090000006
PubMed: 23582365
Anahtar Kelimeler
Immunohistochemistry, Monocrotaline, NF-kappa B, Pulmonary arterial hypertension, Pyrrolidine dithiocarbamate
Kaynak
Pathology Research And Practice
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
209
Sayı
5
