Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

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dc.contributor.authorİmamoğlu, Nalan
dc.contributor.authorEröz, Recep
dc.contributor.authorCanatan, Halit
dc.contributor.authorDemirtaş, Halil
dc.contributor.authorSaatçi, Çetin
dc.date.accessioned2020-04-30T23:19:45Z
dc.date.available2020-04-30T23:19:45Z
dc.date.issued2016
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000371092600001en_US
dc.descriptionPubMed: 26748985en_US
dc.description.abstractDown syndrome (DS) is one of the most common chromosomal disorders. The factors contributing to the mental retardation together with other defects in this syndrome have not been fully explained. Individuals with DS have extra rRNA gene family since they carry an extra chromosome 21. The few reports available are on the relationship between the nucleolus organizer regions (NORs) and DS phenotype. The in vivo regulation of NORs expression on the extra chromosome 21 is not completely understood. Previous studies have shown that nucleoli of lymphocytes from infants (mostly neonates) with DS contain more in vivo and in vitro nucleolar AgNOR proteins when compared with healthy infants. The objective of this study is to compare the in vivo nuclear AgNOR protein level in nucleoplasms (also called as karyoplasm) of nonstimulated peripheral blood lymphocytes from babies/children with DS and healthy controls. Peripheral blood samples obtained from 20 babies/children with DS and 20 matched healthy controls were smeared on clean glass slides and then AgNOR staining was performed. The AgNOR protein level in nucleoplasms of lymphocytes from both groups was calculated using a computer program. Nearly 100 interphase nuclei per individual were analysed. Average nuclear AgNOR protein levels in nucleoplasms of lymphocytes from babies/children with DS were found to be significantly higher than those of the controls (P<0.001). On the basis of our present results, we propose that the increase of nuclear AgNOR protein in in vivo conditions may contribute to the formation of DS phenotypes. Microsc. Res. Tech. 79:133-139, 2016. (c) 2016 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipEURF (Erciyes University Research Fund)Erciyes University [HBMYO-07-02]en_US
dc.description.sponsorshipThe authors are grateful to Mustafa Akgul for the linguistic support in preparing the manuscript. This research was supported by EURF (Erciyes University Research Fund; Contact grant number HBMYO-07-02).en_US
dc.identifier.doi10.1002/jemt.22613en_US
dc.identifier.endpage139en_US
dc.identifier.issn1059-910X
dc.identifier.issn1097-0029
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage133en_US
dc.identifier.urihttps://doi.org/10.1002/jemt.22613
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3842
dc.identifier.volume79en_US
dc.identifier.wosWOS:000371092600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofMicroscopy Research And Techniqueen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectargyrophilic NOR proteinsen_US
dc.subjectimage analysisen_US
dc.subjectnonstimulated lymphocytesen_US
dc.subjectTrisomy 21en_US
dc.titleNuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndromeen_US
dc.typeArticleen_US

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