Development and Validation of Gas Chromatography - Mass Spectrometry Method for Determination of Aldrin and Dieldrin in Serum
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Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
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Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: Exposure to many banned pesticides continues all over the world and in our country due to the long half-lives of pesticides. Therefore, the purpose of the study was to obtain a rapid, simple, and convenient method for the simultaneous determination of aldrin and dieldrin. Material and Methods: Method improving and validation assessment are the most important elements for establishing reference techniques and reliable analysis results. In order to analyze analytes, validation of the analytical method is carried out by checking the parameters of specificity, recovery, precision, linearity, the limit of detection (LOD), and the limit of quantification (LOQ). Results: In our study, an easily applicable, so rapid, effective, and safe GC-MS method was created for the determination of aldrin and dieldrin in serum. In the developed method, 4,4'-Dichlorobenzophenone (4,4-DBP) was used as the internal standard. Aldrin and dieldrin were analyzed in serum in a very short time of 4 minutes. Linear correlation coefficients (R2 ? 0.99) of the linear calibration curves between a range of 1-250 ng/mL of analytes in serum were established. The limit of detection for aldrin and dieldrin were 0.28 and 0.29 ng/mL, respectively. More than 80% recovery of aldrin and dieldrin were obtained at high concentrations, and consistent relative standard deviation (RSD ? 6.03%) values were found for both pesticides. Conclusion: This new method can reliably and quickly be used in routine analysis. The study showed that exposure to aldrin and dieldrin can be detected and monitored in such a short time as four minutes.
Açıklama
Anahtar Kelimeler
dieldrin, Aldrin dieldrin
Kaynak
Sağlık Bilimlerinde Değer (Online)
WoS Q Değeri
Scopus Q Değeri
Cilt
12
Sayı
2
