Effect of combined anti-inflammatory and antioxidant therapy on ischemia-reperfusion injury in rat ovary: an experimental study

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dc.contributor.authorKaya, Murat
dc.contributor.authorÖzkan, Aybars
dc.contributor.authorEröz, Recep
dc.contributor.authorSoyer, Tutku
dc.contributor.authorKabaklıoğlu, Murat
dc.contributor.authorOktay, Murat
dc.contributor.authorKüçük, Adem
dc.date.accessioned2020-05-01T09:11:37Z
dc.date.available2020-05-01T09:11:37Z
dc.date.issued2016
dc.departmentDÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.descriptionSoyer, Tutku/0000-0003-1505-6042en_US
dc.descriptionWOS: 000377959900105en_US
dc.description.abstractAn experiemental study was performed to evaluate the effect of combined anti-inflammatory and antioxidant theraphy on ischemia-reperfusion injury in rat ovary. Also the results of combined theraphy is aimed to compared with monotherapy. Fifty-four female Wistar rats were randomly divided into 9 equal groups (n=6). In Sham group right ovaries of the rats were sampled without generating ischemia and reperfusion injury via median laparatomy. Ischemia/Reperfusion (I/R) was performed by clamping the vascular supply of right ovary for 3 hours (I/R 1 group) and 6 hours (I/R 2 group), respectively. After one hour reperfusion, rats recieved 20 mg/kg Methylprednisolone (Pred 1; 3 hours ischemia and Pred 2; 6 hours ischemia) and 50 mg/kg Vitamin C (Vit C 1; 3 hours ischemia and Vit C 2; 6 hours ischemia). The combined therapy groups (Pred+Vit C 1 and Pred+Vit C 2) were adminstered same doses of both Methylprednisolone and Vitamin C. Rats were sacrifed after 24 hours of reperfusion and ovarian tissues were sampled for oxidative markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)] biochemically. Histopathological findings of inflammation (follicular cell degeneration, vascular congestion, hemorrhage and infiltration by inflammatory cells) were also evaluated with an injury score grading normal findings to severe injury (Grade 0 to 3). The results were compared among groups. Mean levels of antioxidant enzymes and histopathologic grades showed significant difference among groups (P<0.05). MDA and CAT levels were lower in Pred+Vit C 1 than Pred 1, Vit C 1 and I/R 1 (P<0.05). SOD and CAT levels were lower in Pred+Vit C 2 than Pred 2 and I/R 2. Total injury scores were lower in Pred+Vit C 1 and Pred+Vit C 2 than I/R 1 and I/R 2 (P<0.05). The combined treatment of anti-oxidant and anti-inflammatory theraphy reduces the biochemical and histopathologic findings of I/R injury in rat ovary. These results are significantly comparable with the effect of monotheraphy.en_US
dc.identifier.endpage6254en_US
dc.identifier.issn1940-5901
dc.identifier.issue3en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage6246en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5687
dc.identifier.volume9en_US
dc.identifier.wosWOS:000377959900105en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherE-Century Publishing Corpen_US
dc.relation.ispartofInternational Journal Of Clinical And Experimental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIschemia-reperfusionen_US
dc.subjectmethyl prednisoloneen_US
dc.subjectvitamin C and ovaryen_US
dc.titleEffect of combined anti-inflammatory and antioxidant therapy on ischemia-reperfusion injury in rat ovary: an experimental studyen_US
dc.typeArticleen_US

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