Radiofrequency Induced Time-Dependent Alterations in Gene Expression and Apoptosis in Glioblastoma Cell Line

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dc.authoridTuysuz, Mehmet Zahid/0000-0002-2650-5475
dc.authoridSenturk, Fatih/0000-0002-2436-3362;
dc.contributor.authorTuysuz, Mehmet Zahid
dc.contributor.authorKayhan, Handan
dc.contributor.authorSaglam, Atiye Seda Yar
dc.contributor.authorSenturk, Fatih
dc.contributor.authorBagriacik, Emin Umit
dc.contributor.authorYagci, Munci
dc.contributor.authorCanseven, Ayse Gulnihal
dc.date.accessioned2025-10-11T20:48:50Z
dc.date.available2025-10-11T20:48:50Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThe widespread use of wireless communication technologies has increased human exposure to radiofrequency electromagnetic fields (RF-EMFs). Considering the brain's close proximity to mobile phones and its entirely electrical transmission network, it emerges as the organ most profoundly impacted by the RF field. This study aims to investigate the potential effects of RF radiation on cell viability, apoptosis, and gene expressions in glioblastoma cells (U118-MG) at different exposure times (1, 24, and 48 h). To achieve this, we designed and implemented an in vitro RF exposure system operating at a frequency of 2.1 GHz, specifically for cell culture studies, with an average specific absorption rate (SAR) of 1.12 +/- 0.18 W/kg determined through numerical dosimetry calculations. Results reveal a significant influence of a 48 h exposure to a 2.1 GHz RF field on U118-MG cell viability, gene expression, and the induction of caspase (CASP) dependent apoptosis. Notably, increased CASP3, CASP8, and CASP9 mRNA levels were observed after 24 and 48 h of RF treatment. However, only the 48 h RF exposure resulted in apoptotic cell death and a significant elevation in the BAX/BCL-2 ratio. This observed effect may be influenced by extended exposure durations surpassing the cell's doubling time. The increased BAX/BCL-2 ratio, which acts as a key switch for apoptosis, and the heterogeneous morphology of the astrocyte-derived U118-MG cell line may also play a role in this effect.en_US
dc.description.sponsorshipThe authors received no specific funding for this work.en_US
dc.description.sponsorshipThe authors declared that no funding is associated with the study described in this article. The authors would like to thank Melek Yaman and Zubeyir Elmazoglu for their assistance during the experiment.en_US
dc.identifier.doi10.1002/bem.22543
dc.identifier.issn0197-8462
dc.identifier.issn1521-186X
dc.identifier.issue1en_US
dc.identifier.pmid39810728en_US
dc.identifier.scopus2-s2.0-85215307727en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/bem.22543
dc.identifier.urihttps://hdl.handle.net/20.500.12684/22133
dc.identifier.volume46en_US
dc.identifier.wosWOS:001395894200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBioelectromagneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectapoptosisen_US
dc.subjectglioblastomaen_US
dc.subjectnonionizing radiationen_US
dc.subjectradiofrequency electromagnetic fieldsen_US
dc.subjectRF-EMFen_US
dc.titleRadiofrequency Induced Time-Dependent Alterations in Gene Expression and Apoptosis in Glioblastoma Cell Lineen_US
dc.typeArticleen_US

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