Dose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytes

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dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorHatipoğlu, Ömer Faruk
dc.contributor.authorYaykaşlı, Emine
dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorÖzşahin, Mustafa
dc.contributor.authorUslu, Mustafa
dc.date.accessioned2020-05-01T09:11:30Z
dc.date.available2020-05-01T09:11:30Z
dc.date.issued2017
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionYaykasli, Emine/0000-0001-6471-0106; Yaykasli, Kursat/0000-0001-7550-6370; Hatipoglu, Omer Faruk/0000-0002-1012-001Xen_US
dc.descriptionWOS: 000407091600002en_US
dc.descriptionPubMed: 28766346en_US
dc.description.abstractOBJECTIVE: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), comprising of 19 members is a family of peptidases. They have several vital functions in physiological and pathological processes in organisms. ADAMTS-9 has aggrecanolytic activity and is responsible for degradation of aggrecan mainly in articular cartilage. It is known that adiponectin is the most abundantly secreted adipokine (adipocytokines), and the characteristics of adiponectin have not been elucidated yet. It was assumed that adiponectin has anti-inflammatory effect before. However, an inflammatory feature of adiponectin was shown in researches. In our study, the effect of adiponectin on ADAMTS-9 gene expression in primary human chondrocytes was investigated. METHODS: Primary human chondrocytes were exposed to adiponectin at 1, 4, 8 and 12 mu g/ml doses for certain time period. Total RNA was isolated and reverse-transcribed by random primer after incubation. ADAMTS-9 and beta-actin genes expression levels were determined using real-time polymerase chain reaction (qRT-PCR). RESULTS: The highest upregulation of ADAMTS-9 gene expression level was found at 12 mu g/ml dose of adiponectin and 48 h incubation. CONCLUSION: Adiponectin is the key element in the maintenance of cartilage homeostasis. Similarly, the involvement of adiponectin in articular inflammatory diseases was demonstrated in detail. These findings bring adiponectin into central place in the research to develop adiponectin based new therapy methods for arthritic diseases. Together with these findings, our results suggest that adiponectin may be involved in the degradation of articular cartilage by increasing ADAMTS-9 gene expression (Tab. 1, Fig. 3, Ref. 35). Text in PDF www.elis.sk.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technical Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG/111S218]en_US
dc.description.sponsorshipThis study was supported by TUBITAK (The Scientific and Technical Research Council of Turkey), (Project Number: SBAG/111S218).en_US
dc.identifier.doi10.4149/BLL_2017_075en_US
dc.identifier.endpage390en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage386en_US
dc.identifier.urihttps://doi.org/10.4149/BLL_2017_075
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5629
dc.identifier.volume118en_US
dc.identifier.wosWOS:000407091600002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherComenius Univen_US
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectadiponectinen_US
dc.subjectADAMTS-9en_US
dc.subjecthuman chondrocytesen_US
dc.titleDose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytesen_US
dc.typeArticleen_US

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