Sıçan İnce Barsak İskemi/Reperfüzyon Hasarında İleum ve Akciğer Dokusunda Görülen Damar Dışına Protein Kaçışının, Kanabinoid 2 Reseptör Agonisti (Am-1241) ile Kontrolü
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2020
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info:eu-repo/semantics/openAccess
Özet
Amaç: Kanabinoid 2 reseptör agonistinin İskemi/Reperfüzyon (İ/R) hasarı modelinde antiinflamatuvaretkisinin olup olmadığını kanıtlamaktır.Gereç ve Yöntem: Araştırmada; sıçanlarda barsak iskemi ve reperfüzyon modeli oluşturuldu.Kanabinoid 2 reseptör agonisti (AM-1241), iskemi ve reperfüzyon oluşturmadan hemen önceabdominal venden (iv) verildi. Sonrasında evans mavisi iv olarak uygulandı. Dokulara evansmavisinin geçişi çıplak gözle görüldü. Bu aşamadan hemen sonra sıçanın göğüs kafesi açıldı vesistemik kan dolaşım havuzu usulüne uygun olarak boşaltıldı. Dokular tartıldıktan sonra 48 saatformamidde inkübasyona bırakıldı ve spektrofotometrede 620 nm dalgaboyunda ölçüm yapıldı.Bulgular: İ/R grubu şam kontrol grubunu göre yaklaşık % 803 evans mavisi kaçışı izlendi. İ/Rve İ/R+CB2 agonist arasındaki fark ise agonistin proteinleri tutup, protein ve evans mavisinindoku sıvısına geçişini azalttığı görülür.Sonuç: Kanabinoid 2 reseptör agonistinin, hem ileum dokusunda ve hem de uzak organda(akciğer) kılcal damarlardan dokuya protein kaçışını engellediği ve dolayısıyla ileum İ/Rhasarında antiinflamatuar etki gösterdiği bulundu.
Objective: To prove whether the cannabinoid 2 receptor agonist has an anti-inflammatory effect in the model of Ischemia/Reperfusion (I/R) injury. Methods: Intestinal ischemia and reperfusion model was created in rats. The cannabinoid 2 receptor agonist (AM-1241) was given through the abdominal vein (iv) just before creating ischemia and reperfusion. Afterwards, evans blue was applied iv. The transition of evans blue to the tissues was seen with the naked eye. Immediately after this stage, the systemic blood circulation pool was emptied properly. Tissues were incubated at formamide for 48 hours after weighing, and measurements were made at a wavelength of 620 nm on a spectrophotometer. Results: About 803% evans blue escape was observed in the I/R group compared to the sham control group. The difference between the I/R and the I/R+CB2 agonist is that the agonist holds proteins and reduces the transition of protein and evans blue to tissue fluid. Conclusions: The cannabinoid 2 receptor agonist was found to inhibit the escape of protein from both the ileum and the distant organ (lung) capillaries, and thus showed antiinflammatory effect in the ileum I/R injury.
Objective: To prove whether the cannabinoid 2 receptor agonist has an anti-inflammatory effect in the model of Ischemia/Reperfusion (I/R) injury. Methods: Intestinal ischemia and reperfusion model was created in rats. The cannabinoid 2 receptor agonist (AM-1241) was given through the abdominal vein (iv) just before creating ischemia and reperfusion. Afterwards, evans blue was applied iv. The transition of evans blue to the tissues was seen with the naked eye. Immediately after this stage, the systemic blood circulation pool was emptied properly. Tissues were incubated at formamide for 48 hours after weighing, and measurements were made at a wavelength of 620 nm on a spectrophotometer. Results: About 803% evans blue escape was observed in the I/R group compared to the sham control group. The difference between the I/R and the I/R+CB2 agonist is that the agonist holds proteins and reduces the transition of protein and evans blue to tissue fluid. Conclusions: The cannabinoid 2 receptor agonist was found to inhibit the escape of protein from both the ileum and the distant organ (lung) capillaries, and thus showed antiinflammatory effect in the ileum I/R injury.
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