Irradiated riboflavin over nonradiated one: Potent antimigratory, antiproliferative and cytotoxic effects on glioblastoma cells

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dc.authoridHacioglu, Ceyhan/0000-0002-0993-6118en_US
dc.authoridKAR, FATIH/0000-0001-8356-9806en_US
dc.authorscopusid57200116491en_US
dc.authorscopusid57208671422en_US
dc.authorscopusid57208669078en_US
dc.contributor.authorKacar, Sedat
dc.contributor.authorHacioglu, Ceyhan
dc.contributor.authorKar, Fatih
dc.date.accessioned2024-08-23T16:04:15Z
dc.date.available2024-08-23T16:04:15Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractRiboflavin is a water-soluble yellowish vitamin and is controversial regarding its effect on tumour cells. Riboflavin is a powerful photosensitizer that upon exposure to radiation, undergoes an intersystem conversion with molecular oxygen, leading to the production of ROS. In the current study, we sought to ascertain the impact of irradiated riboflavin on C6 glioblastoma cells regarding proliferation, cell death, oxidative stress and migration. First, we compared the proliferative behaviour of cells following nonradiated and radiated riboflavin. Next, we performed apoptotic assays including Annexin V and caspase 3, 7 and 9 assays. Then we checked on oxidative stress and status by flow cytometry and ELISA kits. Finally, we examined inflammatory change and levels of MMP2 and SIRT1 proteins. We caught a clear antiproliferative and cytotoxic effect of irradiated riboflavin compared to nonradiated one. Therefore, we proceeded with our experiments using radiated riboflavin. In all apoptotic assays, we observed a dose-dependent increase. Additionally, the levels of oxidants were found to increase, while antioxidant levels decreased following riboflavin treatment. In the inflammation analysis, we observed elevated levels of both pro-inflammatory and anti-inflammatory cytokines. Additionally, after treatment, we observed reduced levels of MMP2 and SIRT. In conclusion, radiated riboflavin clearly demonstrates superior antiproliferative and apoptotic effects on C6 cells at lower doses compared to nonradiated riboflavin.en_US
dc.identifier.doi10.1111/jcmm.18288
dc.identifier.issn1582-1838
dc.identifier.issn1582-4934
dc.identifier.issue8en_US
dc.identifier.pmid38597418en_US
dc.identifier.scopus2-s2.0-85190147578en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1111/jcmm.18288
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14131
dc.identifier.volume28en_US
dc.identifier.wosWOS:001199476100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Cellular And Molecular Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectapoptosisen_US
dc.subjectcaspase 3, 7 and 9en_US
dc.subjectglioblastoma cellsen_US
dc.subjectinflammationen_US
dc.subjectmetalloproteinasesen_US
dc.subjectoxidative stressen_US
dc.subjectriboflavinen_US
dc.subjectSIRT1en_US
dc.subjectOxidative Stressen_US
dc.subjectProliferationen_US
dc.subjectCanceren_US
dc.subjectSurvivalen_US
dc.subjectInvasionen_US
dc.subjectP53en_US
dc.titleIrradiated riboflavin over nonradiated one: Potent antimigratory, antiproliferative and cytotoxic effects on glioblastoma cellsen_US
dc.typeArticleen_US

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