Honeybee biodiversity based on five mitochondrial genes and new evidence for different sublineages in Türkiye
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the genetic structure of honey bee populations in T & uuml;rkiye was analyzed using eleven restriction endonuclease enzymes targeting the COI-COII, COI, 16S rDNA, ND5 and CYT B genes. A total of 553 worker honey bee samples were collected from 43 different districts across T & uuml;rkiye, as well as from Greece, Bulgaria, and Georgia countries neighboring T & uuml;rkiye. Analysis of the COI region using the Hin fI restriction enzyme revealed the presence of two mitotypes. Type 1 was the predominant type in T & uuml;rkiye, while type 2 was detected exclusively in Hatay, a province in the southern part of the country. Notably, the absence of the HincII site in the COI region and the EcoRI site in the 16S rDNA gene in the Hatay samples suggests that these samples belong to the A lineage. However, several samples from Hatay were classified as belonging to mitochondrial lineage O due to the presence of BglII and Hin fI restriction sites in the CYT B gene. Except for Hatay, which displayed an EcoRI site in the 16S rDNA fragment and lacked a Hin fI site in the CYT B fragment, all samples were identified as belonging to the C lineage. Furthermore, the COI-COII restriction patterns with DraI for lineages C and Z were consistent with the findings. The restriction analysis revealed that several samples from Hatay exhibited the most divergent mitotype in comparison with other honey bee populations studied, resembling African or Arabian honey bees. The results of this study indicate that Anatolia serves as a genetic center for Middle Eastern honey bees, and Hatay acts as a transitional zone. However, the findings also suggest that the introduction of foreign commercial queen bees into T & uuml;rkiye has led to some degree of hybridization between native and nonnative honey bee subspecies.
Açıklama
Anahtar Kelimeler
Honey bee, mtDNA, PCR-RFLP, T & uuml;rkiye
Kaynak
Turkish Journal of Veterinary & Animal Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
48
Sayı
6
