Conjunctival Impression Cytology by Using a Thermosensitive Adhesive: Polymerized N-isopropyl Acrylamide

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dc.contributor.authorTunç, Murat
dc.contributor.authorYıldırım, Ümran
dc.contributor.authorYüksel, Harun
dc.contributor.authorCheng, Xuanhong
dc.contributor.authorHumayun, Mark
dc.contributor.authorRatner, Buddy
dc.date.accessioned2020-04-30T22:41:39Z
dc.date.available2020-04-30T22:41:39Z
dc.date.issued2009
dc.departmentDÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000268624300011en_US
dc.descriptionPubMed: 19574910en_US
dc.description.abstractPurpose: We aimed to evaluate a new technique using a thermosensitive glue coating for impression cytology of the ocular Surface. Methods: We prepared plasma polymerized N-isopropyl acrylamide (pNIPAM)-coated parylene C (poly(monochloro-p-xylylene)) films to obtain cytological samples from the conjunctival surface. We compared this new technique with the conventional nitrocellulose paper method in regards to the quality of impression cytology and quantity of the cells in 30 postmenauposal women with dry eye complaints. Results: The cellular material was adequate for evaluation in 28 (93%) of 30 eyes in pNIPAM-coated parylene group and in 18 (60%) of 30 eyes in the nitrocellulose group (P = 0.0002). pNIPAM-coated parylene technique was superior to the nitrocellulose technique regarding the mean number of cells per microscopic field (P = 0.00003), integrity of the cells (P = 0.00001), and cellular preservation (P = 0.0002). Conclusion: The number of cells and the quality scores were significantly higher in the pNIPAM-coated impression cytology technique than the nitrocellulose method.en_US
dc.identifier.doi10.1097/ICO.0b013e318196706den_US
dc.identifier.endpage773en_US
dc.identifier.issn0277-3740
dc.identifier.issn1536-4798
dc.identifier.issue7en_US
dc.identifier.startpage770en_US
dc.identifier.urihttps://doi.org/10.1097/ICO.0b013e318196706d
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3226
dc.identifier.volume28en_US
dc.identifier.wosWOS:000268624300011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofCorneaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectimpression cytologyen_US
dc.subjectpNIPAMen_US
dc.subjectdry eyeen_US
dc.titleConjunctival Impression Cytology by Using a Thermosensitive Adhesive: Polymerized N-isopropyl Acrylamideen_US
dc.typeArticleen_US

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