Dermal absorption and toxicity of alpha amanitin in mice

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dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorSürmen, Mustafa Gani
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorKarahan, Selim
dc.contributor.authorOktay, Murat
dc.contributor.authorTuran, Hakan
dc.contributor.authorErdem, Havva
dc.date.accessioned2020-05-01T09:11:18Z
dc.date.available2020-05-01T09:11:18Z
dc.date.issued2014
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionYaykasli, Kursat/0000-0001-7550-6370; Kaya, Ertugrul/0000-0003-0081-682Xen_US
dc.descriptionWOS: 000336731700015en_US
dc.descriptionPubMed: 23763309en_US
dc.description.abstractThe fungus Amanita phalloides is known to contain two main groups of toxins: amanitins and phallotoxins. The amanitins group effectively blocks the RNA polymerase II enzyme found in eukaryotic cells. As alpha amanitin has a lethal effect on the majority of eukaryotic cells, it can be valuable as an antiparasitic or antifungal drug. It can be used externally against ectoparasites. It is critical that percutaneous applications of the alpha amanitin toxin are not harmful to the recipient. In this study, the absorption and the toxicity of percutaneous and intraperitoneal (ip) applications of 1 mg/kg alpha amanitin to mice were compared. Potential skin, liver and kidney toxicities were investigated through pathological examination. HPLC analysis was used to determine the amount of the toxin. No toxicity or toxin were found in the skin, liver, or kidneys of the mice in the control group. Interestingly, the percutaneous application group also showed no toxicity, and the toxin was not present in this group. After 24 h, Councilman-like bodies and pyknotic cells were observed in the mice in which alpha amanitin was applied intraperitoneally, demonstrating the presence of toxicity. Peak levels of alpha amanitin (mu g/mL) in the liver, kidney, and blood in the ip application group were measured at 3.3 (6 h), 0.2 (6 h) and 1.2 (1 h), respectively. The results demonstrated that the toxin was not absorbed through the skin of the mice and that the percutaneous application of alpha amanitin did not have any toxic effects. Thus, alpha amanitin may be administered percutaneously for therapeutic purposes.en_US
dc.description.sponsorshipDuzce University Scientific Research Coordinator (Duzce Universitesi Bilimsel Arastirma Projeleri Koordinatorlugu, DUBAP)Duzce University [2011.04.HD.042]en_US
dc.description.sponsorshipThis study was financially supported by a research grant from Duzce University Scientific Research Coordinator (Duzce Universitesi Bilimsel Arastirma Projeleri Koordinatorlugu, DUBAP, 2011.04.HD.042).en_US
dc.identifier.doi10.3109/15569527.2013.802697en_US
dc.identifier.endpage160en_US
dc.identifier.issn1556-9527
dc.identifier.issn1556-9535
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage154en_US
dc.identifier.urihttps://doi.org/10.3109/15569527.2013.802697
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5479
dc.identifier.volume33en_US
dc.identifier.wosWOS:000336731700015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.relation.ispartofCutaneous And Ocular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha amanitinen_US
dc.subjectdermal absorptionen_US
dc.subjectdermal toxicityen_US
dc.subjectHPLCen_US
dc.titleDermal absorption and toxicity of alpha amanitin in miceen_US
dc.typeArticleen_US

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