Concanavalin A induces apoptosis in a dose-dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells

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dc.authoridKacar, Sedat/0000-0002-0671-8529
dc.authoridkar, fatih/0000-0001-8356-9806
dc.authoridSAHINTURK, VAROL/0000-0003-2317-3644
dc.authoridHacioglu, Ceyhan/0000-0002-0993-6118
dc.authorwosidKacar, Sedat/I-2544-2019
dc.authorwosidkar, fatih/AAN-4285-2021
dc.authorwosidkar, fatih/AAI-4540-2021
dc.authorwosidSAHINTURK, VAROL/V-5195-2017
dc.contributor.authorKar, Fatih
dc.contributor.authorKacar, Sedat
dc.contributor.authorHacioglu, Ceyhan
dc.contributor.authorKanbak, Gungor
dc.contributor.authorSahinturk, Varol
dc.date.accessioned2021-12-01T18:49:30Z
dc.date.available2021-12-01T18:49:30Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractGlioma is the most common brain tumor. C6 rat glioblastoma cells provide the possibility to the scientist to study brain cancer. Concanavalin A (Con A) has a lot of antitumoral effects, especially over oxidative stress. In the present study, it was aimed to decide the impacts of various doses of Con A on C6 glioblastoma cells regarding cytotoxicity, thiol/disulfide homeostasis, apoptosis, and inflammation. We detected the cytotoxic activity of Con A (from 7.8 to 500 mu g/ml) in C6 cells by utilizing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and determined the toxic concentration of Con A. Once the optimal doses were found, the thiol-disulfide homeostasis, levels of total antioxidant and oxidant status (TAS and TOS), malondialdehyde (MDA) and glutathione (GSH), pro-inflammatory cytokines as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), apoptotic proteins as cytochrome c (CYCS), and caspase 3 (CASP3) were measured. Apoptotic and morphological changes in the C6 cells were examined with an inverted microscope and flow cytometry technique. Dose-dependent Con A triggered oxidative damage in the C6 cells, affecting the inflammatory pathway, so reducing proliferation with apoptotic proteins and morphological changes. But especially, Con A increased disulfide formation by disrupting the thiol/disulfide balance in C6 cells. This study revealed that Con A, known as carbohydrate-binding protein, generated oxidative damage, inflammation, and apoptosis in a dose-dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells.en_US
dc.description.sponsorshipCommission of Scientific Research Projects of Eskisehir Osmangazi University, Eskisehir, Turkey [2019-1915]en_US
dc.description.sponsorshipThis study was supported by the Commission of Scientific Research Projects of Eskisehir Osmangazi University, Eskisehir, Turkey, with the grant numbers: 2019-1915.en_US
dc.identifier.doi10.1002/jbt.22742
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85100988754en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/jbt.22742
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10733
dc.identifier.volume35en_US
dc.identifier.wosWOS:000619382000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Biochemical And Molecular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbrain tumoren_US
dc.subjectcanceren_US
dc.subjectcell deathen_US
dc.subjectconcanavalin Aen_US
dc.subjectcytotoxicityen_US
dc.subjectoxidative stressen_US
dc.titleConcanavalin A induces apoptosis in a dose-dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cellsen_US
dc.typeArticleen_US

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