The dual role of boron in vitro neurotoxication of glioblastoma cells via SEMA3F/NRP2 and ferroptosis signaling pathways

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dc.authoridHacıoğlu, Ceyhan/0000-0002-0993-6118
dc.authoridKAR, FATIH/0000-0001-8356-9806
dc.contributor.authorKar, Fatih
dc.contributor.authorHacıoğlu, Ceyhan
dc.contributor.authorKaçar, Sedat
dc.date.accessioned2023-07-26T11:57:16Z
dc.date.available2023-07-26T11:57:16Z
dc.date.issued2023
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.description.abstractGlioblastoma multiform (GBM) is a malignant tumor cancer that originates from the star-shaped glial support tissues, namely astrocytes, and it is associated with a poor prognosis in the brain. The GBM has no cure, and chemotherapy, radiation therapy, and immunotherapy are all ineffective. A certain dose of Boric acid (BA) has many biochemical effects, conspicuously over antioxidant/oxidant rates. This article sought to investigate the modifies of various doses of BA on the glioblastoma concerning cytotoxicity, ferroptosis, apoptosis, and semaphorin-neuropilin signaling pathway. The Cytotoxic activity and cell viability of BA (0.39-25 mM) in C6 cells were tested at 24, 48, and 72 h using 3-(4,5-dimethylthiazol, 2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The IC50 concentration of BA at 1.56 mM was found and cell lysate used for biochemical analysis. Glutathione peroxidase 4 (GPx4) and ACLS4 levels of ferroptosis, levels of total antioxidant (TAS) and oxidant (TAS) parameters, malondialdehyde (MDA), apoptotic proteins as caspase 3 (CASP3) and caspase 7 (CASP7) were measured. The ferroptosis, semaphoring-neuropilin, apoptotic pathway markers and cell counts were analyzed with flow cytometry, Q-PCR, Western and Elisa technique in the C6 cell lysate. BA triggered ferroptosis in the C6 cells dose-dependently, affecting the semaphorin pathway, so reducing proliferation with apoptotic compared with untreated cell as control group (p < .05). This study revealed that BA, defined as trace element and natural compound, incubated ferroptosis, total oxidant molecules, and caspase protein in a dose-dependently by disrupting SEMA3F in tumor cells.en_US
dc.description.sponsorshipCommission of Scientific Research Projects of Eskisehir Osmangazi University [2019-1915]en_US
dc.description.sponsorshipThis study was supported by the Commission of Scientific Research Projects of Eskisehir Osmangazi University, Eskisehir, Turkey with the grant numbers 2019-1915.en_US
dc.identifier.doi10.1002/tox.23662
dc.identifier.endpage77en_US
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.issue1en_US
dc.identifier.pmid36136913en_US
dc.identifier.scopus2-s2.0-85138544959en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://doi.org/10.1002/tox.23662
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13100
dc.identifier.volume38en_US
dc.identifier.wosWOS:000856259500001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorHacıoğlu, Ceyhan
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEnvironmental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectBoric Acid; Brain Tumor; Cancer; Cytotoxicity; Ferroptosis; Semaphorinen_US
dc.subjectSemaphorinsen_US
dc.titleThe dual role of boron in vitro neurotoxication of glioblastoma cells via SEMA3F/NRP2 and ferroptosis signaling pathwaysen_US
dc.typeArticleen_US

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