Reduction of the argyrophilic nucleolar organizing region associated protein synthesis with age in buccal epithelial cells of healthy individuals

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dc.contributor.authorSelvi, Burak
dc.contributor.authorDemirtaş, Halil
dc.contributor.authorEröz, Recep
dc.contributor.authorİmamoğlu, Nalan
dc.date.accessioned2020-04-30T23:31:35Z
dc.date.available2020-04-30T23:31:35Z
dc.date.issued2015
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000351531100012en_US
dc.descriptionPubMed: 25082567en_US
dc.description.abstractBackground Nucleolus organizer regions (NORs) consist of the rRNA coding gene family (rDNA) in the cell nucleus. The argyrophilic proteins are selectively stained with silver nitrate and bind these regions. It was reported that NOR (rDNA) activity decreases in human lymphocytes, fibroblasts and bone marrow with age. However, to our knowledge there have not been any studies related to the NORs in oral epithelial cells of healthy individuals. Aim Our aim is to detect any correlation between age and Total AgNOR area/Total nucleus area (TAA/TNA) values in buccal epithelial cells of healthy individuals. Methods Oral epithelial cells from 50 healthy individuals (age range of 2-80 years old) were spread onto a clean glass slide, air dried and fixed. Then the AgNOR staining protocol was performed on these cells. TAA/TNA ratio and AgNOR dots were calculated using software. From each person 50 oral epithelial cells were evaluated. Results Statistically significant correlations were found between mean TAA/TNA values and age (Rsq = 0.534, p < 0.001 for linear and Rsq = 0.728, p < 0.0001 for polynominal regression), and between AgNOR number and age (Rsq = 0.621, p < 0.001 for linear and Rsq = 0.693, p < 0.0001 for polynominal regression). Conclusion There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.en_US
dc.description.sponsorshipErciyes University Research FundErciyes University [TSY-08-559]; Medical Research Council of Erciyes University (ERUBAP)Erciyes University [TSY-08-559]en_US
dc.description.sponsorshipErciyes University Research Fund has supported this research; contract Grant Number: TSY-08-559. This study is the essential part of the MS thesis of Burak Selvi. The Medical Research Council of Erciyes University (ERUBAP, Project No. TSY-08-559) supported this work.en_US
dc.identifier.doi10.1007/s40520-014-0263-6en_US
dc.identifier.endpage208en_US
dc.identifier.issn1594-0667
dc.identifier.issn1720-8319
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage201en_US
dc.identifier.urihttps://doi.org/10.1007/s40520-014-0263-6
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4329
dc.identifier.volume27en_US
dc.identifier.wosWOS:000351531100012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAging Clinical And Experimental Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNORen_US
dc.subjectAgNORsen_US
dc.subjectBuccal epithelial cellsen_US
dc.subjectrDNAen_US
dc.subjectNucleolusen_US
dc.titleReduction of the argyrophilic nucleolar organizing region associated protein synthesis with age in buccal epithelial cells of healthy individualsen_US
dc.typeArticleen_US

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