Small non-coding RNAs have predicted roles in reproductive biology and transposable element regulation in the parasitic worm Strongyloides venezuelensis

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dc.authoridReynolds, Kieran/0009-0000-6543-4545
dc.authoridKikuchi, Taisei/0000-0003-2759-9167
dc.contributor.authorLastik, Dominika
dc.contributor.authorKounosu, Asuka
dc.contributor.authorDayi, Mehmet
dc.contributor.authorYoshida, Akemi
dc.contributor.authorFujihira, Akito
dc.contributor.authorReynolds, Kieran
dc.contributor.authorHunt, Vicky L.
dc.date.accessioned2025-10-11T20:48:24Z
dc.date.available2025-10-11T20:48:24Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThe gastrointestinal parasitic nematode Strongyloides spp. has a unique life cycle that alternates between a parasitic generation that reproduces through mitotic parthenogenesis and a dioecious free-living sexually reproducing generation. Adult females from these two generations are genetically identical, making them an informative model to identify molecular differences between parasitic and free-living lifestyles and understand different reproductive strategies. We investigated the expression of small RNAs (sRNAs) that are either enriched for a 5' monophosphate modification (5'pN) or are 5' modification-independent, across five life cycle stages of the rodent parasite Strongyloides venezuelensis. We identified miRNAs and small-interfering RNAs expressed by S. venezuelensis that are predicted to target and regulate the expression of protein-coding genes and transposable elements (TEs). Three previously unreported classes of sRNA were identified: (1) 25Gs with a putative role in reproduction in adult females, (2) tRNA-derived 24-28 nt sRNAs (tsRNAs) which are predicted to target TEs in free-living females, and (3) 5'pN-enriched 26-29Cs with 5' CGAATCC and 3' TTT motifs expressed in parasitic females. We also confirmed that S. venezuelensis expresses the 27G class of sRNAs involved in TE regulation, which was previously identified in the rodent parasite Strongyloides ratti. Taken together, these results provide new insights into the role of sRNAs in reproductive biology and parasitism.en_US
dc.description.sponsorshipJapanese Society for the Promotion of Scienceen_US
dc.description.sponsorshipThe authors thank all members of Parasite Systems Biology lab for their critical comments and technical supports. Computations were partially performed on the NIG supercomputer at ROIS National Institute of Genetics.en_US
dc.identifier.doi10.1038/s41598-025-01968-2
dc.identifier.issn2045-2322
dc.identifier.issue1en_US
dc.identifier.pmid40593876en_US
dc.identifier.scopus2-s2.0-105009721476en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-025-01968-2
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21910
dc.identifier.volume15en_US
dc.identifier.wosWOS:001522988300015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNature Portfolioen_US
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectStrongyloides venezuelensisen_US
dc.subjectSmall RNAen_US
dc.subjectmicroRNAen_US
dc.subjectHelminthen_US
dc.subjectNematodeen_US
dc.subjectParasiteen_US
dc.titleSmall non-coding RNAs have predicted roles in reproductive biology and transposable element regulation in the parasitic worm Strongyloides venezuelensisen_US
dc.typeArticleen_US

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