The effect of the enzymes trypsin and DNase I on the antimicrobial efficiency of root canal irrigation solutions

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dc.authoridÇankaya, Tülin Doğan/0000-0003-0607-6703en_US
dc.authoridErdonmez, Demet/0000-0002-5331-2206en_US
dc.authoridUGUR AYDIN, ZELIHA/0000-0002-1773-9114en_US
dc.authorscopusid57810201400en_US
dc.authorscopusid57204392771en_US
dc.authorscopusid56049522100en_US
dc.authorwosidÇankaya, Tülin Doğan/AAU-1848-2021en_US
dc.contributor.authorCankaya, Tuelin Dogan
dc.contributor.authorAydin, Zeliha Ugur
dc.contributor.authorErdonmez, Demet
dc.date.accessioned2024-08-23T16:07:12Z
dc.date.available2024-08-23T16:07:12Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThe purpose of this study was to evaluate the antibacterial efficacy of using 2.5% NaOCl, 2% chlorhexidine (CHX), Irritrol, and chitosan-coated silver nanoparticles (AgCNPs) alone or in combination with deoxyribonuclease I (DNase I) and trypsin pre-enzyme applications in dentin samples contaminated with Enterococcus faecalis (E. faecalis) by CLSM. 144 dentin blocks with confirmed E. faecalis biofilm formation were divided randomly according to the irrigation protocol (n = 12): NaOCl, CHX, Irritrol, AgCNPs, trypsin before NaOCl, CHX, Irritrol, AgCNPs, and DNase I before NaOCl, CHX, Irritrol, AgCNPs. Dentin blocks were stained with the Live/Dead BacLight Bacterial Viability Kit and viewed with CLSM after irrigation applications. The percentage of dead and viable bacteria was calculated using ImageJ software on CLSM images. At a significance level of p < 0.05, the obtained data were analyzed using one-way Anova and post-hoc Tukey tests. In comparison with NaOCl, CHX had a higher percentage of dead bacteria, both when no pre-enzyme was applied and when DNase I was applied as a pre-enzyme (p < 0.05). There was no difference in the percentage of dead bacteria between the irrigation solutions when trypsin was applied as a pre-enzyme (p > 0.05). AgCNPs showed a higher percentage of dead bacteria when trypsin was applied as a pre-enzyme compared to other irrigation solutions (p < 0.05), while the pre-enzyme application did not affect the percentage of dead bacteria in NaOCl, CHX, and Irritrol (p > 0.05). No irrigation protocol tested was able to eliminate the E. faecalis biofilm. While the application of trypsin as a pre-enzyme improved the antimicrobial effect of AgCNPs, it did not make any difference over other irrigation solutions.en_US
dc.description.sponsorshipAbant Izzet Baysal niversitesien_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s10266-023-00894-x
dc.identifier.endpage937en_US
dc.identifier.issn1618-1247
dc.identifier.issn1618-1255
dc.identifier.issue3en_US
dc.identifier.pmid38280114en_US
dc.identifier.scopus2-s2.0-85183401709en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage929en_US
dc.identifier.urihttps://doi.org/10.1007/s10266-023-00894-x
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14539
dc.identifier.volume112en_US
dc.identifier.wosWOS:001150694500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofOdontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDNase Ien_US
dc.subjectE. faecalisen_US
dc.subjectIrritrolen_US
dc.subjectSilver chitosan nanoparticleen_US
dc.subjectTrypsinen_US
dc.subjectEnterococcus-Faecalisen_US
dc.subject2-Percent Chlorhexidineen_US
dc.subjectSilver Nanoparticlesen_US
dc.subjectAntibiofilm Activityen_US
dc.subjectSodium-Hypochloriteen_US
dc.subjectExtracellular Dnaen_US
dc.subjectDiode-Laseren_US
dc.subjectBiofilmen_US
dc.subjectSusceptibilityen_US
dc.subjectDisinfectionen_US
dc.titleThe effect of the enzymes trypsin and DNase I on the antimicrobial efficiency of root canal irrigation solutionsen_US
dc.typeArticleen_US

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