Development and validation of SSR markers for the plant-parasitic nematode Subanguina moxae using genome assembly of Illumina pair-end reads

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dc.contributor.authorTakeuchi, Tomoaki
dc.contributor.authorYamaguchi, Madoka
dc.contributor.authorTanaka, Ryusei
dc.contributor.authorDayı, Mehmet
dc.contributor.authorOgura, Nobuo
dc.contributor.authorKikuchi, Taisei
dc.date.accessioned2020-05-01T09:11:24Z
dc.date.available2020-05-01T09:11:24Z
dc.date.issued2015
dc.departmentDÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.descriptionWOS: 000355666300002en_US
dc.description.abstractSubanguina moxae, belonging to the subfamily Anguininae, is an obligate parasite of Artemisia plants, which are widely used as cooking herbs and in traditional medicine in East Asia. Because the nematode is distributed throughout East Russia and East Asia, there is concern about the potential for significant damage to commercial farming; however, details about its biology remain unclear. To investigate the genetic diversity of S. moxae, we developed 2243 simple sequence repeat (SSR) markers using Illumina short reads of the genomic DNA. We validated 100 randomly selected markers indicating their robustness and examined polymorphisms among nematode populations sampled from four different locations in Japan. These SSR markers will be a useful tool for understanding the population structure and transmission patterns of this parasitic nematode.en_US
dc.description.sponsorshipJapan Society for the Promotion of Science (JSPS) KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [24780044, 26292178]en_US
dc.description.sponsorshipThe authors thank Mitsuteru Akiba for technical assistance. This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant Nos. 24780044 and 26292178).en_US
dc.identifier.doi10.1163/15685411-00002885en_US
dc.identifier.endpage522en_US
dc.identifier.issn1388-5545
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage515en_US
dc.identifier.urihttps://doi.org/10.1163/15685411-00002885
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5561
dc.identifier.volume17en_US
dc.identifier.wosWOS:000355666300002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBrill Academic Publishersen_US
dc.relation.ispartofNematologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArtemisiaen_US
dc.subjectde novo assemblyen_US
dc.subjectgenetic diversityen_US
dc.subjectmicrosatelliteen_US
dc.titleDevelopment and validation of SSR markers for the plant-parasitic nematode Subanguina moxae using genome assembly of Illumina pair-end readsen_US
dc.typeArticleen_US

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