A deep eutectic solvent based liquid phase microextraction for the determination of caffeine in Turkish coffee samples by HPLC-UV
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel liquid-phase microextraction procedure for the determination and extraction of caffeine using a deep eutectic solvent based liquid-phase microextraction method (DES-LPME) was developed. A deep eutectic solvent consisting of choline chloride-phenol (1:3) and tetrahydrofuran as a dilution solvent were used for the extraction of caffeine from Turkish coffee samples. The quantitative recoveries were obtained when the DES volume was 400 mu L, and the volume of tetrahydrofuran that was used as an emulsifier solvent was 800 mu L. The limit of detection and the limit of quantification were found to be 0.12 and 0.40 mu g mL(-1), respectively. Linear dynamic range was 0.5-100 mu g mL(-1), and coefficient of determination (R-2) was 0.9998. The relative standard deviation (RSD) was 2.20% when the standard solution concentration was 1.0 mu g mL(-1). The fact that the determination coefficient obtained from the calibration curve was greater than 0.9998 gives information about the accuracy of the method. Also, in order to determine the accuracy of the developed method, selected coffee samples were spiked with 25 and 50 mu g mL(-1) caffeine.
Açıklama
Anahtar Kelimeler
Caffeine, liquid phase microextraction, deep eutectic solvents, coffee, Food, Water, Chromatography, Extraction, Tea
Kaynak
Food Additives And Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
37
Sayı
3
