Identification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stress

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dc.contributor.authorAkbudak, M. Aydın
dc.contributor.authorFiliz, Ertuğrul
dc.contributor.authorUylaş, Senem
dc.date.accessioned2020-04-30T23:18:24Z
dc.date.available2020-04-30T23:18:24Z
dc.date.issued2019
dc.departmentDÜ, Çilimli Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionUylas, Senem/0000-0002-7689-1677; Akbudak, M. Aydin/0000-0002-1397-4678; Filiz, Ertugrul/0000-0001-9636-6389en_US
dc.descriptionWOS: 000462022300036en_US
dc.descriptionPubMed: 30443823en_US
dc.description.abstractCysteine (Cys) is the first identified molecule in plant metabolism which includes both sulfur and nitrogen. It can be synthesized in three cellular compartments, containing chloroplast, cytoplasm and mitochondrion. The final step of cysteine biosynthesis is catalyzed by the O-acetylserine(thiol)lyase enzyme (OASTL, E.C. 4.2.99). In the present study, seven members of the OASTL gene family in the sorghum (Sorghum bicolor) genome were identified at a genome-wide scale and comparative bioinformatics analyses were performed between sorghum and Arabidopsis OASTLs. In all OASTL proteins, a pyridoxal-phosphate dependent domain structure (PALP, PF00291) was identified. The gene ontology annotations also revealed that all sorghum OASTL genes have KOG1252 (Cystathionine beta-synthase and related enzyme) and K01738 (cysteine synthase A) activities. In promotor sequences of OASTL genes, diverse cis-acting elements were found, including hormone and light responsiveness, abiotic stress responsiveness, and tissue-specific ones (meristem and endosperm). Sorghum OASTL genes demonstrated medium or high level expressions in anatomical parts and developmental stages based on the digital expression data. Expression of OASTL genes were also analyzed under cadmium (Cd) stress in sorghum by Real Time-quantitative PCR (RT-qPCR). The results exclusively showed that OASTL A1-2 gene was 1.12 fold up-regulated in roots, whereas cysteine synthase 26 was 2.25 fold down-regulated in leaves. The predicted 3D structure of OASTLs indicated some structural diversities as well as variations in the secondary structures.en_US
dc.identifier.doi10.1007/s11033-018-4477-0en_US
dc.identifier.endpage354en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage343en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-018-4477-0
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3272
dc.identifier.volume46en_US
dc.identifier.wosWOS:000462022300036en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCysteineen_US
dc.subjectCadmiumen_US
dc.subjectSulfuren_US
dc.subjectSorghumen_US
dc.subjectCo-expressionen_US
dc.subjectBioinformaticsen_US
dc.titleIdentification of O-acetylserine(thiol)lyase (OASTL) genes in sorghum (Sorghum bicolor) and gene expression analysis under cadmium stressen_US
dc.typeArticleen_US

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