EZH2 expression in colorectal carcinoma: an evaluation of clinicopathological and prognostic value

dc.authoridDemir, Fatih/0000-0002-8181-0739;
dc.contributor.authorCagatay, Diren Vuslat
dc.contributor.authorBalci, Mecdi Gurhan
dc.contributor.authorIssin, Gizem
dc.contributor.authorDemir, Fatih
dc.date.accessioned2025-10-11T20:48:40Z
dc.date.available2025-10-11T20:48:40Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractBackgroundEnhancer of zeste homolog 2 (EZH2), the catalytic subunit of Polycomb Repressive Complex 2, catalyzes trimethylation of histone H3 lysine 27 and has been implicated in tumor progression.AimOur study aims to assess the relationship between EZH2 expression and clinicopathologic data, and survival in colorectal carcinomas (CRC).Materials and methodsEZH2 immunohistochemistry was performed on tumor blocks from 124 CRC patients. Based on H-scores, cases were stratified into low- and high-expression groups. EZH2 status was correlated with demographic, clinical, and pathologic features, and overall survival was analyzed with the Kaplan-Meier method and log-rank test.ResultsA total of 124 cases of CRC; 12 (9.7%) cases were classified as low EZH2 expression, and 112 (90.3%) cases were classified as high EZH2 expression. A significant association was found between EZH2 expression and microsatellite stability. High EZH2 expression was significantly enriched in microsatellite-stable tumors (p = 0.007) and in left-sided lesions (p = 0.049). No significant associations were detected with sex, stage, grade, lymph-node status, or vascular invasion. Kaplan-Meier analysis revealed no difference in overall survival between low- and high-EZH2 groups (log-rank p = 0.47; hazard ratio = 1.13, 95% CI 0.45-2.85). EZH2 staining was uniformly strong in normal mucosa and adenomas, mirroring the high expression observed in most CRCs.ConclusionsEZH2 is highly expressed across the normal-adenoma-carcinoma sequence and, in our cohort, was not linked to overall survival or to most clinicopathologic parameters in CRC. The lower expression observed in a subset of MSI-H tumors warrants further investigation; present evidence remains insufficient to establish EZH2 as an independent prognostic biomarker.en_US
dc.description.sponsorshipErzincan University Scientific Research and Project Office [910]en_US
dc.description.sponsorshipThis study was supported by Erzincan University Scientific Research and Project Office (Project ID:910).en_US
dc.identifier.doi10.1007/s12672-025-02990-6
dc.identifier.issn2730-6011
dc.identifier.issue1en_US
dc.identifier.pmid40536699en_US
dc.identifier.scopus2-s2.0-105008712395en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s12672-025-02990-6
dc.identifier.urihttps://hdl.handle.net/20.500.12684/22043
dc.identifier.volume16en_US
dc.identifier.wosWOS:001512205900007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofDiscover Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectColorectal carcinomaen_US
dc.subjectEZH2en_US
dc.subjectHistone Methyl transferaseen_US
dc.subjectPrognosisen_US
dc.subjectMicrosatelliteen_US
dc.titleEZH2 expression in colorectal carcinoma: an evaluation of clinicopathological and prognostic valueen_US
dc.typeArticleen_US

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