The Efficacy of Electrochemotherapy with Dacarbazine on Melanoma Cells

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dc.authoridSenturk, Fatih/0000-0002-2436-3362en_US
dc.authorscopusid57188561538en_US
dc.authorscopusid16241261700en_US
dc.authorscopusid57223847596en_US
dc.authorscopusid37072233300en_US
dc.authorscopusid10639904600en_US
dc.authorwosidSenturk, Fatih/AEI-3613-2022en_US
dc.contributor.authorCoskun, Alaaddin
dc.contributor.authorKayhan, Handan
dc.contributor.authorSenturk, Fatih
dc.contributor.authorEsmekaya, Meric Arda
dc.contributor.authorCanseven, Ayse Gulnihal
dc.date.accessioned2024-08-23T16:04:21Z
dc.date.available2024-08-23T16:04:21Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractElectrochemotherapy (ECT) involves locally applying electrical pulses to permeabilize cell membranes, using electroporation (EP). This process enhances the uptake of low-permeant chemotherapeutic agents, consequently amplifying their cytotoxic effects. In melanoma treatment, dacarbazine (DTIC) is a cornerstone, but it faces limitations because of poor cell membrane penetration, necessitating the use of high doses, which, in turn, leads to increased side effects. In our study, we investigated the effects of DTIC and EP, both individually and in combination, on the melanoma cell line (SK-MEL-30) as well as human dermal fibroblasts (HDF) using in vitro assays. First, the effects of different DTIC concentrations on the viability of SK-MEL-30 and HDF cells were determined, revealing that DTIC was more effective against melanoma cells at lower concentrations, whereas its cytotoxicity at 1000 mu M was similar in both cell types. Next, an ideal electric field strength of 1500 V/cm achieved a balance between permeability (84%) and melanoma cell viability (79%), paving the way for effective ECT. The combined DTIC-EP (ECT) application reduced IC50 values by 2.2-fold in SK-MEL-30 cells and 2.7-fold in HDF cells compared with DTIC alone. In conclusion, ECT not only increased DTIC's cytotoxicity against melanoma cells but also affected healthy fibroblasts. These findings emphasize the need for cautious, targeted ECT management in melanoma therapy.en_US
dc.description.sponsorshipGazi University [01/2015-03]en_US
dc.description.sponsorshipThe research was supported by the Scientific Research Projects (01/2015-03) at Gazi University. The authorsexpress their gratitude for the assistance provided by Dr.Ongun Onaran, Dr. Sanem Ozcelik, and Dr. Mehmed ZahidTuysuz.en_US
dc.identifier.doi10.1089/bioe.2023.0041
dc.identifier.endpage125en_US
dc.identifier.issn2576-3105
dc.identifier.issn2576-3113
dc.identifier.issue2en_US
dc.identifier.pmid39119570en_US
dc.identifier.scopus2-s2.0-85194493990en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage118en_US
dc.identifier.urihttps://doi.org/10.1089/bioe.2023.0041
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14176
dc.identifier.volume6en_US
dc.identifier.wosWOS:001201389200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofBioelectricityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectelectrochemotherapyen_US
dc.subjectdacarbazineen_US
dc.subjectmelanoma cell linesen_US
dc.subjecthuman dermal fibroblastsen_US
dc.subjectMalignant-Melanomaen_US
dc.subjectPharmacokineticsen_US
dc.titleThe Efficacy of Electrochemotherapy with Dacarbazine on Melanoma Cellsen_US
dc.typeArticleen_US

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